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However, available exporters are scarce, due to the limited knowledge of bacterial transporters. Here, we built a workflow for exporter mining and devised a tunable plug\u2010and\u2010play exporter (TuPPE) module to improve the production of macrolide biopesticides in\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    . Combining genome analyses and experimental confirmations, we found three ATP\u2010binding cassette transporters that contribute to milbemycin production in\n                    <jats:italic>Streptomyces bingchenggensis<\/jats:italic>\n                    . We then optimized the expression level of target exporters for milbemycin titer optimization by designing a TuPPE module with replaceable promoters and ribosome binding sites. Finally, broader applications of the TuPPE module were implemented in industrial\n                    <jats:italic>S. bingchenggensis<\/jats:italic>\n                    BC04,\n                    <jats:italic>Streptomyces avermitilis<\/jats:italic>\n                    NEAU12 and\n                    <jats:italic>Streptomyces cyaneogriseus<\/jats:italic>\n                    NMWT1, which led to optimal titer improvement of milbemycin A3\/A4, avermectin B\n                    <jats:sub>1a<\/jats:sub>\n                    and nemadectin \u03b1 by 24.2%, 53.0% and 41.0%, respectively. Our work provides useful exporters and a convenient TuPPE module for titer improvement of macrolide biopesticides in\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    . More importantly, the feasible exporter mining workflow developed here might shed light on widespread applications of exporter engineering in\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    to boost the production of other secondary metabolites.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.13883","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T12:46:16Z","timestamp":1629981976000},"page":"1120-1132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Mining and engineering exporters for titer improvement of macrolide biopesticides in\n                    <i>Streptomyces<\/i>"],"prefix":"10.1111","volume":"15","author":[{"given":"Liyang","family":"Chu","sequence":"first","affiliation":[{"name":"School of Life Science Northeast Agricultural University No. 59 Mucai Street, Xiangfang District Harbin 150030 China"},{"name":"State Key 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