{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:23:51Z","timestamp":1791591831128,"version":"4.3.4"},"reference-count":97,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Sofosbuvir and Daclatasvir are among the direct\u2010acting antiviral (DAA) medications prescribed for the treatment of chronic hepatitis C (CHC) virus infection as combination therapy with other antiviral medications. DAA\u2010based therapy achieves high cure rates, reaching up to 97% depending on the genotype of the causative hepatitis C virus (HCV). While DAAs have been approved as an efficient and well\u2010tolerated therapy for CHC, emerging concerns about adverse cardiac side effects, higher risk of recurrence and occurrence of hepatocellular carcinoma (HCC) and doubts of genotoxicity have been reported. In our study, we investigated in detail physiological off\u2010targets of DAAs and dissected the effects of these drugs on cellular organelles using budding yeast, a unicellular eukaryotic organism. DAAs were found to disturb the architecture of the endoplasmic reticulum (ER) and the mitochondria, while showing no apparent genotoxicity or DNA damaging effect. Our study provides evidence that DAAs are not associated with genotoxicity and highlights the necessity for adjunctive antioxidant therapy to mitigate the adverse effects of DAAs on ER and mitochondria.<\/jats:p>","DOI":"10.1111\/1751-7915.13904","type":"journal-article","created":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T03:47:12Z","timestamp":1628653632000},"page":"2199-2213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Profiling the physiological pitfalls of anti\u2010hepatitis C direct\u2010acting agents in budding yeast"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9466-7863","authenticated-orcid":false,"given":"Galal","family":"Yahya","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology Faculty of Pharmacy Zagazig University Al Sharqia 44519 Egypt"},{"name":"Department of Molecular Genetics Faculty of Biology Technical University of Kaiserslautern Paul\u2010Ehrlich Str. 24 Kaiserslautern 67663 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nashwa","family":"Hashem Mohamed","sequence":"additional","affiliation":[{"name":"Hospitals of Zagazig University Al Sharqia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0350-6376","authenticated-orcid":false,"given":"Jordi","family":"Pijuan","sequence":"additional","affiliation":[{"name":"Laboratory of Neurogenetics and Molecular Medicine \u2010 IPER Institut de Recerca Sant Joan de D\u00e9u Barcelona 08950 Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noura M.","family":"Seleem","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Faculty of Pharmacy Zagazig University Al Sharqia 44519 Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha","family":"Mosbah","sequence":"additional","affiliation":[{"name":"Infection Control Unit Hospitals of Zagazig University Al Sharqia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Faculty of Biology Technical University of Kaiserslautern Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"Abdelmoaty","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine Faculty of Medicine Zagazig University Zagazig 44519 Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafa","family":"Almeer","sequence":"additional","affiliation":[{"name":"Department of Zoology College of Science King Saud University P.O. 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