{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:40:34Z","timestamp":1780450834661,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075\u201015\u20102019\u20101880"],"award-info":[{"award-number":["075\u201015\u20102019\u20101880"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The gut microbiota plays a significant role in human health; however, the complex relationship between gut microbial communities and host health is still to be thoroughly studied and understood. Microbes in the distal gut contribute to host health through the biosynthesis of vitamins and essential amino acids and the generation of important metabolic by\u2010products from dietary components that are left undigested by the small intestine. Aged citrus peel (Chenpi) is used in traditional Chinese medicine to lower cholesterol, promote weight loss and treat various gastrointestinal symptoms. This study investigated how the microbial community changes during treatment with Chenpi using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). Two preparations of Chenpi extract were tested: Chenpi suspended in oil only and Chenpi in a viscoelastic emulsion. Short\u2010chain fatty acids (SCFAs) were measured during treatment to monitor changes in the microbial community of the colon presenting a decrease in production for acetic, propionic and butyric acid (ANOVA (\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.001) during the 15\u2009days of treatment. 16S rRNA sequencing of microbial samples showed a clear difference between the two treatments at the different sampling times (ANOSIM\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.003; ADOSIM\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u20090.002 [\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u200969%]). Beta diversity analysis by PcoA showed differences between the two Chenpi formulations for treatment day 6. These differences were no longer detectable as soon as the Chenpi treatment was stopped, showing a reversible effect of Chenpi on the human microbiome. 16S rRNA sequencing of microbial samples from the descending colon showed an increase in\n                    <jats:italic>Firmicutes<\/jats:italic>\n                    for the treatment with the viscoelastic emulsion. At the genus level,\n                    <jats:italic>Roseburia<\/jats:italic>\n                    ,\n                    <jats:italic>Blautia<\/jats:italic>\n                    ,\n                    <jats:italic>Subdoligranulum<\/jats:italic>\n                    and\n                    <jats:italic>Eubacterium<\/jats:italic>\n                    increased in numbers during the viscoelastic emulsion treatment. This study sheds light on the anti\u2010obesity effect of a polymethoxyflavone (PMFs)\u2010enriched Chenpi extract and creates a foundation for the identification of \u2018obesity\u2010prevention\u2019 biomarkers in the gut microbiota.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14005","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T06:55:56Z","timestamp":1644994556000},"page":"874-885","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Anti\u2010obesity effects of Chenpi: an artificial gastrointestinal system study"],"prefix":"10.1111","volume":"15","author":[{"given":"Maria","family":"Falduto","sequence":"first","affiliation":[{"name":"New Jersey Institute for Food, Nutrition and Health Rutgers State University New Brunswick NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Smedile","sequence":"additional","affiliation":[{"name":"Department of Marine and Coastal Sciences Rutgers State University New Brunswick NJ USA"},{"name":"Institute of Polar Science Italian National Research Council Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Food Science Rutgers State University New Brunswick NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zheng","sequence":"additional","affiliation":[{"name":"Health Promoting Naturals Laboratory School of Environmental and Biological Sciences Rutgers State University New Brunswick NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Food Science Rutgers State University New Brunswick NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingrong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Food Science Rutgers State University New Brunswick NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2109-2912","authenticated-orcid":false,"given":"Richard","family":"Weeks","sequence":"additional","affiliation":[{"name":"Health Promoting Naturals Laboratory School of Environmental and Biological Sciences Rutgers State University New Brunswick NJ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey M.","family":"Ermakov","sequence":"additional","affiliation":[{"name":"Agrobiotechnology Center Don State Technical University Rostov\u2010on\u2010Don Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Chikindas","sequence":"additional","affiliation":[{"name":"Health Promoting Naturals Laboratory School of Environmental and Biological Sciences Rutgers State University New Brunswick NJ USA"},{"name":"Agrobiotechnology Center Don State Technical University Rostov\u2010on\u2010Don Russia"},{"name":"I.M. Sechenov First Moscow State Medical University Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11112690"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9993.2001.01070.pp.x"},{"key":"e_1_2_9_4_1","first-page":"65","article-title":"Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population","volume":"59","author":"Andoh A.","year":"2016","journal-title":"J Clin BiochemNutr"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2008.613"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02449"},{"key":"e_1_2_9_7_1","first-page":"197","article-title":"Modulation of gut microbiota by berberine improves steatohepatitis in high\u2010fat diet\u2010Fed BALB\/C mice","volume":"19","author":"Cao Y.","year":"2016","journal-title":"Arch Iran Med"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9993.1993.tb00438.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.01.035"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12010016"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2008.0107"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-48382-5_1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/ilar\/ilv021"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2009.00712.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5b06157"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026284"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2019.10.015"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2015-309990"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.577474"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00228615"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02291"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002530051022"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3810\/pgm.2009.11.2074"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093827"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.702559"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1219"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2584"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00775"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01541-09"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.10.020"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.10.020"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2016-0130"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.01982.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2013.12.010"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00759-10"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41366-019-0332-1"},{"key":"e_1_2_9_39_1","doi-asserted-by":"crossref","unstructured":"Wang Q. Garrity G.M. Tiedje J.M. Cole J.C. Wang Q. Tiedje J.M. et\u00a0al. (2007)Na\u00efve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy.https:\/\/doi.org\/10.1128\/AEM.00062\u201067","DOI":"10.1128\/AEM.00062-07"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphereDirect.00073-17"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9FO02907A"},{"key":"e_1_2_9_42_1","first-page":"1","article-title":"Modulation of gut microbiota by berberine and metformin during the treatment of high\u2010fat diet\u2010induced obesity in rats","volume":"5","author":"Zhang X.U.","year":"2015","journal-title":"Sci Rep"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11102291"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14005","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.14005","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T17:00:08Z","timestamp":1762275608000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.14005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,16]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1111\/1751-7915.14005"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.14005","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,16]]},"assertion":[{"value":"2021-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}