{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T21:45:57Z","timestamp":1792014357925,"version":"4.4.0"},"reference-count":58,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T00:00:00Z","timestamp":1644883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31602080"],"award-info":[{"award-number":["31602080"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31860691"],"award-info":[{"award-number":["31860691"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013066","name":"Key Scientific Research Project of Colleges and Universities in Henan Province","doi-asserted-by":"publisher","award":["21A230015"],"award-info":[{"award-number":["21A230015"]}],"id":[{"id":"10.13039\/501100013066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Protective efficiency of a combination of four recombinant\n                    <jats:italic>Brucella abortus<\/jats:italic>\n                    (\n                    <jats:italic>B. abortus<\/jats:italic>\n                    ) proteins, namely, ribosomal protein L7\/L12, outer membrane protein (OMP) 22, OMP25 and OMP31, was evaluated as a combined subunit vaccine (CSV) against\n                    <jats:italic>B. abortus<\/jats:italic>\n                    infection in RAW 264.7 cell line and murine model. Four proteins were cloned, expressed and purified, and their immunocompetence was analysed. BALB\/c mice were immunized subcutaneously with single subunit vaccines (SSVs) or CSV. Cellular and humoral immune responses were determined by ELISA. Results of immunoreactivity showed that these four recombinant proteins reacted with\n                    <jats:italic>Brucella<\/jats:italic>\n                    \u2010positive serum individually but not with\n                    <jats:italic>Brucella<\/jats:italic>\n                    \u2010negative serum. A massive production of IFN\u2010\u03b3 and IL\u20102 but low degree of IL\u201010 was observed in mice immunized with SSVs or CSV. In addition, the titres of IgG2a were heightened compared with IgG1 in SSV\u2010 or CSV\u2010immunized mice, which indicated that SSVs and CSV induced a typical T\u2010helper\u20101\u2010dominated immune response\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    . Further investigation of the CSV showed a superior protective effect in mice against brucellosis. The protection level induced by CSV was significantly higher than that induced by SSVs, which was not significantly different compared with a group immunized with RB51. Collectively, these antigens of\n                    <jats:italic>Brucella<\/jats:italic>\n                    could be potential candidates to develop subunit vaccines, and the CSV used in this study could be a potential candidate therapy for the prevention of brucellosis.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14015","type":"journal-article","created":{"date-parts":[[2022,2,15]],"date-time":"2022-02-15T03:46:41Z","timestamp":1644896801000},"page":"1811-1823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Immunization with a combination of recombinant\n                    <i>Brucella abortus<\/i>\n                    proteins induces T helper immune response and confers protection against wild\u2010type challenge in BALB\/c mice"],"prefix":"10.1111","volume":"15","author":[{"given":"Zhiqiang","family":"Li","sequence":"first","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuli","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Life Sciences Henan Normal University  Xinxiang Henan Province 453007 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangli","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xi","sequence":"additional","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinliang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfang","family":"Hao","sequence":"additional","affiliation":[{"name":"College of Biology and Food Shangqiu Normal University  Shangqiu Henan Provence 476000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shihezi University  Shihezi Xinjiang Provence 832003 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4132-0006","authenticated-orcid":false,"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Shihezi University  Shihezi Xinjiang Provence 832003 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-090110-102905"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/743509"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2003.11.001"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00211-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2015.05.057"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-016-2076-x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2016.02.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00110-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.3.1130-1133.1995"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2018.e01079"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2012.02.126"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2018.10.002"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/abr.abr_10_17"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2015.04.015"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9020152"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftaa002"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192439399"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00391"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.04.019"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnv003"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104137"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.577026"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.JJID.2017.019"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4823-4830.2001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.3.717"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.01.043"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.08.052"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-017-2405-4"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.5.2734-2741.2006"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.2.1080"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-021-02190-0"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00213"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2012.03.011"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12550-009-0033-z"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-2074-7"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874318801004010102"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.11.5201-5204.1994"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016203"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2017.12.014"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9314-9"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00211.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2019-0261"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-009-0123-8"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02421-6"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.04.009"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00255-9"},{"key":"e_1_2_9_48_1","first-page":"44","article-title":"Design and production of a novel polypeptide with immunogenic potentials for immunoassay of Brucella melitensis","volume":"2","author":"Sharghi S.H.","year":"2017","journal-title":"Trends Peptide Protein Sci"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2019.151362"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602-020-09684-1"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2017.10.013"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.3.979-989.1982"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.09.015"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2018.12.007"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1108.041197"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2782-2786.1996"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12065"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.4238\/2015.October.5.10"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00576"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14015","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.14015","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14015","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:27:54Z","timestamp":1762280874000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.14015"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,15]]},"references-count":58,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1111\/1751-7915.14015"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.14015","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,15]]},"assertion":[{"value":"2021-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}