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We constructed a six tandem repeat of lactoferricin cDNA driven by promoter PtrnQ. After transformation, two transformants P245 and P263 possessing a stable inheritance of plasmid and high expression of lactoferricin were selected. The bactericidal activities, 1\u2009\u03bcl of aliquot of a total 5.5\u2009ml of solution extracted from 5\u2009ml of cultured P245 and P263, were equivalent to the efficacy of 238.25 and 322.7\u2009ng of Ampicillin against\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , respectively, and 366.4 and 452.52\u2009ng of Ampicillin against\n                    <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                    respectively. These extracts were able to kill an Ampicillin\u2010resistant\n                    <jats:italic>E.\u00a0coli<\/jats:italic>\n                    strain. The bactericidal activities of P245 and P263 equivalent to the efficacy of Tetracycline against\n                    <jats:italic>Vibrio parahaemolyticus<\/jats:italic>\n                    and\n                    <jats:italic>V. alginolyticus<\/jats:italic>\n                    were also determined. Moreover, the bactericidal activities of P245 and P263 were 168.04 and 249.94\u2009ng of Ampicillin against\n                    <jats:italic>Edwardsiella tarda<\/jats:italic>\n                    , respectively, and 219.7 and 252.43\u2009ng of Tetracycline against\n                    <jats:italic>Streptococcus iniae<\/jats:italic>\n                    respectively. Interestingly, the survival rate of\n                    <jats:italic>E. tarda<\/jats:italic>\n                    \u2010infected tilapia fry fed the P263 extract displayed a significantly greater than that of the fry\u2010fed control strain. Collectively, these\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>subtilis<\/jats:italic>\n                    transgenic strains are highly promising for use in animal husbandry during a disease outbreak.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14026","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T03:16:11Z","timestamp":1646277371000},"page":"1895-1909","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["High antimicrobial activity of lactoferricin\u2010expressing\n                    <i>Bacillus subtilis<\/i>\n                    strains"],"prefix":"10.1111","volume":"15","author":[{"given":"Bing\u2010Chang","family":"Lee","sequence":"first","affiliation":[{"name":"Institute of Biomedical Sciences Mackay Medical College  New Taipei City Taiwan"},{"name":"Liver Disease Prevention and Treatment Research 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