{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T11:54:17Z","timestamp":1792065257645,"version":"4.4.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["3332021092"],"award-info":[{"award-number":["3332021092"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Coronavirus disease 2019 (COVID\u201019), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS\n                    <jats:italic>\u2010<\/jats:italic>\n                    CoV\n                    <jats:italic>\u2010<\/jats:italic>\n                    2), has recently posed a significant threat to global public health. The objective of this study was to develop and evaluate a rapid, expandable and sequencing\u2010free high\u2010resolution melting (HRM) approach for the direct detection and classification of SARS\u2010CoV\u20102. Thirty\u2010one common pathogens that can cause respiratory tract infections were used to evaluate the specificity of the method. Synthetic RNA with serial dilutions was utilized to determine the sensitivity of the method. Finally, the clinical performance of the method was assessed using 290 clinical samples. The one\u2010step multiplex HRM could accurately identify SARS\u2010CoV\u20102 and differentiate mutations in each marker site within approximately 2\u2009h. For each target, the limit of detection was lower than 10 copies\/reaction, and no cross\u2010reactivity was observed among organisms within the specificity testing panel. The method showed good uniformity for SARS\u2010CoV\u20102 detection with a consistency of 100%. Regarding the clade classification performance, the results showed good concordance compared with sequencing, with the rate of agreement being 95.1% (78\/82). The one\u2010step multiplex HRM method is a rapid method for SARS\u2010CoV\u20102 detection and classification.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14027","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T00:28:47Z","timestamp":1646180927000},"page":"1883-1894","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection and classification of SARS\u2010CoV\u20102 using high\u2010resolution melting analysis"],"prefix":"10.1111","volume":"15","author":[{"given":"Liying","family":"Sun","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"},{"name":"Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing 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