{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T11:52:34Z","timestamp":1792324354367,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172321"],"award-info":[{"award-number":["82172321"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    is an important opportunistic human pathogen, which raises a worldwide concern for its increasing resistance. Nonthermal plasma, which is also called cold atmospheric plasma (CAP), is an alternative therapeutic approach for clinical infectious diseases. However, the bacterial factors that affect CAP treatment remain unclear. The sterilization effect of a portable CAP device on different\n                    <jats:italic>P.\u2009aeruginosa<\/jats:italic>\n                    strains was investigated in this study. Results revealed that CAP can directly or indirectly kill\n                    <jats:italic>P.\u2009aeruginosa<\/jats:italic>\n                    in a time\u2010dependent manner. Scanning electron microscopy and transmission electron microscope showed negligible surface changes between CAP\u2010treated and untreated\n                    <jats:italic>P.\u2009aeruginosa<\/jats:italic>\n                    cells. However, cell leakage occurred during the CAP process with increased bacterial lactate dehydrogenase release. More importantly, pigmentation of the\n                    <jats:italic>P.\u2009aeruginosa<\/jats:italic>\n                    culture was remarkably reduced after CAP treatment. Further mechanical exploration was performed by utilizing mutants with loss of functional genes involved in pyocyanin biosynthesis, including\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    PAO1 strain\u2010derived\n                    <jats:italic>phzA1<\/jats:italic>\n                    ::Tn,\n                    <jats:italic>phzA2<\/jats:italic>\n                    ::Tn, \u0394\n                    <jats:italic>phzA1<\/jats:italic>\n                    \/\u0394\n                    <jats:italic>phzA2<\/jats:italic>\n                    ,\n                    <jats:italic>phzM<\/jats:italic>\n                    ::Tn and\n                    <jats:italic>phzS<\/jats:italic>\n                    ::Tn, as well as corresponding gene deletion mutants based on clinical PA1 isolate. The results indicated that pyocyanin and its intermediate 5\u2010methyl phenazine\u20101\u2010carboxylic acid (5\u2010Me\u2010PCA) play important roles in\n                    <jats:italic>P.\u2009aeruginosa<\/jats:italic>\n                    resistance to CAP treatment. The unique enzymes, such as PhzM in the pyocyanin biosynthetic pathway, could be novel targets for the therapeutic strategy design to control the growing\n                    <jats:italic>P.\u2009aeruginosa<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14032","type":"journal-article","created":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T16:38:56Z","timestamp":1647362336000},"page":"1910-1921","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Pyocyanin biosynthesis protects\n                    <i>Pseudomonas aeruginosa<\/i>\n                    from nonthermal plasma inactivation"],"prefix":"10.1111","volume":"15","author":[{"given":"Huyue","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Pharmacy The Second Affiliated Hospital Army Medical University  Chongqing 400037 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