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In general, the production of 1, 3\u2010PDO by wild\u2010type\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    is relatively low. Therefore, a new gene manipulation of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    was developed to improve the production of 1, 3\u2010PDO by overexpressing in the reduction pathway and attenuating the by\u2010products in the oxidation pathway. Firstly,\n                    <jats:italic>dhaB<\/jats:italic>\n                    and\/or\n                    <jats:italic>dhaT<\/jats:italic>\n                    were overexpressed in the reduction pathway. Considering the cost of IPTG, the constitutive promoter P32 was selected to express the key gene. By comparing\n                    <jats:italic>K.P<\/jats:italic>\n                    . pET28a\u2010P32\u2010\n                    <jats:italic>dhaT<\/jats:italic>\n                    with the original strain, the production of 1, 3\u2010PDO was increased by 19.7%, from 12.97 to 15.53\u2009g\u2009l\n                    <jats:sup>\u22121<\/jats:sup>\n                    (in a 250\u2009ml shaker flask). Secondly, three\n                    <jats:italic>lldD<\/jats:italic>\n                    and\n                    <jats:italic>budC<\/jats:italic>\n                    regulatory sites were selected in the by\u2010product pathway, respectively, using the CRISPR\u2010dCas9 system, and the optimal regulatory sites were selected following the 1, 3\u2010PDO production. As a result, the 1, 3\u2010PDO production by\n                    <jats:italic>K.P<\/jats:italic>\n                    . L1\u2010pRH2521 and\n                    <jats:italic>K.P<\/jats:italic>\n                    . B3\u2010pRH2521 reached up to 19.16 and 18.74\u2009g\u2009l\n                    <jats:sup>\u22121<\/jats:sup>\n                    , which was increased by 47.7% and 44.5% respectively. Overexpressing\n                    <jats:italic>dhaT<\/jats:italic>\n                    and inhibiting expression of\n                    <jats:italic>lldD<\/jats:italic>\n                    and\n                    <jats:italic>budC<\/jats:italic>\n                    were combined to further enhance the ability of\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    to produce 1, 3\u2010PDO. The 1, 3\u2010PDO production by\n                    <jats:italic>K.P<\/jats:italic>\n                    . L1\u2010B3\u2010PRH2521\u2010P32\u2010\n                    <jats:italic>dhaT<\/jats:italic>\n                    reached 57.85\u2009g\u2009l\n                    <jats:sup>\u22121<\/jats:sup>\n                    in a 7.5\u2009l fermentation tank (with Na\n                    <jats:sup>+<\/jats:sup>\n                    neutralizer), which is higher than that of the original strain. This is the first time that the 1, 3\u2010PDO production was improved in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    by overexpressing the key gene and attenuating by\u2010product synthesis in the CRISPR\u2010dCas9 system. This study reports an efficient approach to regulate the expression of genes in\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    to increase the 1, 3\u2010PDO production, and such a strategy may be useful to modify other strains to produce valuable chemicals.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14033","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T16:30:54Z","timestamp":1647534654000},"page":"2112-2125","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Enhancing the capability of\n                    <i>Klebsiella pneumoniae<\/i>\n                    to produce 1, 3\u2010propanediol by overexpression and regulation through CRISPR\u2010dCas9"],"prefix":"10.1111","volume":"15","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Systems Bioengineering (Ministry of Education) 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Platform Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) School of Chemical Engineering and Technology Tianjin University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Systems Bioengineering (Ministry of Education) Tianjin University  Tianjin 300072 China"},{"name":"SynBio Research Platform Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) School of Chemical Engineering and Technology Tianjin University  Tianjin 300072 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Systems Bioengineering (Ministry of Education) Tianjin University  Tianjin 300072 China"},{"name":"SynBio Research Platform Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) School of Chemical Engineering 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