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To facilitate this effort, we have developed two 96\u2010well plate format colorimetric assays for reliable quantification of various ketones and aldehydes from culture supernatants, based on either a vanillin\u2010acetone reaction or the 2,4\u2010dinitrophenylhydrazine (2,4\u2010DNPH) reagent. The vanillin\u2010acetone assay enabled accurate and selective measurement of acetone titers up to 2\u2009g\u2009l\n                    <jats:sup>\u22121<\/jats:sup>\n                    in a minimal culture medium. The 2,4\u2010DNPH\u2010based assay can be used for a wide range of aldehydes and ketones, shown here through the optimization of conditions for 15 different compounds. Both assays were implemented to improve acetone production from different substrates by an engineered\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    strain. The fast and user\u2010friendly colorimetric assays proposed here open the potential for iterative rounds of (automated) strain and pathway engineering and screening, facilitating the efforts towards further boosting production titers of industrially relevant ketones and aldehydes.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14097","type":"journal-article","created":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T01:41:19Z","timestamp":1654911679000},"page":"2426-2438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["High\u2010throughput colorimetric assays optimized for detection of ketones and aldehydes produced by microbial cell factories"],"prefix":"10.1111","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9370-6157","authenticated-orcid":false,"given":"Ekaterina","family":"Kozaeva","sequence":"first","affiliation":[{"name":"The Novo Nordisk Foundation Center for 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