{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:48:22Z","timestamp":1790628502196,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003339","name":"Consejo Superior de Investigaciones Cient\u00edficas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"publisher","award":["ACIF\/2019\/214"],"award-info":[{"award-number":["ACIF\/2019\/214"]}],"id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ergosterol is a specific sterol component of yeast and fungal membranes. Its biosynthesis is one of the most effective targets for antifungal treatments. However, the emergent resistance to multiple sterol\u2010based antifungal drugs emphasizes the need for new therapeutic approaches. The allylamine terbinafine, which selectively inhibits squalene epoxidase Erg1 within the ergosterol biosynthetic pathway, is mainly used to treat dermatomycoses, whereas its effectiveness in other fungal infections is limited. Given that ergosterol biosynthesis depends on iron as an essential cofactor, in this report, we used the yeast\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    to investigate how iron bioavailability influences Erg1 expression and terbinafine susceptibility. We observed that both chemical and genetic depletion of iron decrease\n                    <jats:italic>ERG1<\/jats:italic>\n                    expression, leading to an increase in terbinafine susceptibility. Deletion of either\n                    <jats:italic>ROX1<\/jats:italic>\n                    transcriptional repressor or\n                    <jats:italic>CTH1<\/jats:italic>\n                    and\n                    <jats:italic>CTH2<\/jats:italic>\n                    post\u2010transcriptional repressors of\n                    <jats:italic>ERG1<\/jats:italic>\n                    expression led to an increase in Erg1 protein levels and terbinafine resistance. On the contrary, overexpression of\n                    <jats:italic>CTH2<\/jats:italic>\n                    led to the opposite effect, lowering Erg1 levels and increasing terbinafine susceptibility. Although strain\u2010specific particularities exist, opportunistic pathogenic strains of\n                    <jats:italic>S.\u00a0cerevisiae<\/jats:italic>\n                    displayed a response similar to the laboratory strain. These data indicate that iron bioavailability and particular regulatory factors could be used to modulate susceptibility to terbinafine.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14102","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T03:22:31Z","timestamp":1657855351000},"page":"2705-2716","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Modulation of yeast Erg1 expression and terbinafine susceptibility by iron bioavailability"],"prefix":"10.1111","volume":"15","author":[{"given":"Tania","family":"Jord\u00e1","sequence":"first","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA) Consejo Superior de Investigaciones Cient\u00edficas (CSIC)  Paterna, Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Mart\u00ednez\u2010Mart\u00edn","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA) Consejo Superior de Investigaciones Cient\u00edficas (CSIC)  Paterna, Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Teresa","family":"Mart\u00ednez\u2010Pastor","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular Universitat de Val\u00e8ncia  Burjassot, Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1856-490X","authenticated-orcid":false,"given":"Sergi","family":"Puig","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de Alimentos (IATA) Consejo Superior de Investigaciones Cient\u00edficas (CSIC)  Paterna, Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1809"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2020.101033"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/169.4.859"},{"key":"e_1_2_8_5_1","volume-title":"CLSI standard M27\u2010A3 (28)","author":"Clinical and Laboratory Standards Institute","year":"2008"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-006-9077-7"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2010.10.025"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1078\/0723202041438473"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2019.02.002"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aap7999"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.00953"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01782-16"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.14611"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M705570200"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.1.6.1041-1044.2002"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1567-1364.12043"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes11070795"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7110901"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.08.051"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139306"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-B"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2016.06.012"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.12.3890-3900.2003"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.01940.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098094"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2020.118797"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2019.02.008"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","unstructured":"Ollinger T.L. 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