{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T12:45:45Z","timestamp":1782909945405,"version":"3.54.5"},"reference-count":53,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T00:00:00Z","timestamp":1661299200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The antibiotic\u2010resistant\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (Kp) has become a significant crisis in treating pneumonia. Low\u2010frequency ultrasound (LFU) is promising to overcome the obstacles. Mice were infected with bioluminescent Kp Xen39 by intratracheal injection to study the therapeutic effect of LFU in combination with antibiotics. The counts per second (CPS) were assessed with an animal biophoton imaging system. Bacterial clearance, histopathology, and the concentrations of cytokines were determined to evaluate the therapeutic effect. LC\u2013MS\/MS was used to detect the distribution of antibiotics in the lung and plasma. LFU in combination with meropenem (MEM) or amikacin (AMK) significantly improved the behavioural state of mice. The CPS of the LFU combination group were more significantly decreased compared with those of the antibiotic alone groups. The average colony\u2010forming units of lung tissue in the LFU combination groups were also lower than those of the antibiotic groups. Although no significant changes of cytokines (IL\u20106 and TNF\u2010\u03b1) in plasma and bronchoalveolar lavage fluid were observed, LFU in combination with antibiotics showed less inflammatory damage from histopathological results compared with the antibiotic\u2010alone groups. Moreover, 10\u00a0min of LFU treatment promoted the distribution of MEM and AMK in mouse lung tissue at 60 and 30\u2009min, respectively, after dosage. LFU could enhance the effectiveness of MEM and AMK in the treatment of Kp\u2010induced pneumonia, which might be attributed to the fact that LFU could promote the distribution of antibiotics in lung tissue and reduce inflammatory injury.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14134","type":"journal-article","created":{"date-parts":[[2022,8,24]],"date-time":"2022-08-24T12:29:36Z","timestamp":1661344176000},"page":"2819-2830","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Synergistic effect of low\u2010frequency ultrasound and antibiotics on the treatment of\n                    <i>Klebsiella pneumoniae<\/i>\n                    pneumonia in mice"],"prefix":"10.1111","volume":"15","author":[{"given":"Kaicheng","family":"Yan","sequence":"first","affiliation":[{"name":"Medical School of Chinese PLA  Beijing China"},{"name":"Department of Pharmacy, Center of Medicine Clinical Research, Medical Supplies Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianli","family":"Yang","sequence":"additional","affiliation":[{"name":"Medical School of Chinese PLA  Beijing China"},{"name":"Department of Pharmacy, Center of Medicine Clinical Research, Medical Supplies Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Center of Medicine Clinical Research, Medical Supplies Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liuhan","family":"Dong","sequence":"additional","affiliation":[{"name":"Medical School of Chinese PLA  Beijing China"},{"name":"Department of Pharmacy, Center of Medicine Clinical Research, Medical Supplies Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Center of Medicine Clinical Research, Medical Supplies Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6687-9984","authenticated-orcid":false,"given":"Yun","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Center of Medicine Clinical Research, Medical Supplies Center Chinese PLA General Hospital  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191788"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00246-20"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2014.01.007"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ptj\/81.7.1351"},{"key":"e_1_2_11_6_1","first-page":"243","article-title":"Multicenter randomized trial comparing meropenem (1.5 g daily) and imipenem\/cilastatin (2 g daily) in the hospital treatment of community\u2010acquired pneumonia","volume":"25","author":"Bartoloni A.","year":"1999","journal-title":"Drugs under Experimental and Clinical Research"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.sap.0000168695.20350.07"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01465-06"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/2317846"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885328204040540"},{"key":"e_1_2_11_11_1","first-page":"796","article-title":"Preliminary study on resistance mechanism and virulence features in carbapenems\u2010resistant Klebsiella pneumoniae from burn patients","volume":"34","author":"Chen Z.","year":"2018","journal-title":"Zhonghua Shao Shang Za Zhi"},{"key":"e_1_2_11_12_1","volume-title":"Performance standards for antimicrobial susceptibility testing, M100","author":"CLSI","year":"2021"},{"key":"e_1_2_11_13_1","first-page":"32","article-title":"The effects of low\u2010frequency ultrasound (35 kHz) on methicillin\u2010resistant Staphylococcus aureus (MRSA) in vitro","volume":"56","author":"Conner\u2010Kerr T.","year":"2010","journal-title":"Ostomy\/Wound Management"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13613-020-00715-2"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199732020-00002"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/39.6.677"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2013.06.006"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13217"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.04.023"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1501049112"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.49.2.104"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03673-1"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060520956473"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00203-19"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01120.2005"},{"key":"e_1_2_11_26_1","first-page":"10","article-title":"Use of ultrasonics and antibiotics in the treatment of wounds in patients with high risk of infection of vascular transplants","volume":"7","author":"Komrakov V.E.","year":"1962","journal-title":"Klinicheskaia Khirurgiia"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.546382"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2009.160"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.3662048"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2015.05.030"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2016.03.016"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-016-0428-x"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63953-3"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2021.06.005"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885066618754784"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux013"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1086\/590064"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2020.07.002"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.11.2577"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.22361"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0206041"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2012.758"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-003-0292-0"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-019-3167-7"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10122698"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix783"},{"key":"e_1_2_11_47_1","first-page":"6023101","article-title":"Multiple low frequency ultrasound enhances bactericidal activity of vancomycin against methicillin\u2010resistant Staphylococcus aureus biofilms","volume":"2018","author":"Wang J.","year":"2018","journal-title":"BioMed Research International"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(21)00399-6"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199550010-00007"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5154.80412"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultsonch.2008.02.001"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00149-19"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.30705"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz446"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14134","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.14134","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14134","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:52:34Z","timestamp":1762271554000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.14134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,24]]},"references-count":53,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/1751-7915.14134"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.14134","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,24]]},"assertion":[{"value":"2022-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}