{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T09:03:10Z","timestamp":1781341390693,"version":"3.54.1"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T00:00:00Z","timestamp":1669075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100019465","name":"Arab-German Young Academy of Sciences and Humanities","doi-asserted-by":"publisher","award":["SSE\u2010PHYS\u2010N.A.\u2010MG\u2010TypeB\u2010FY23"],"award-info":[{"award-number":["SSE\u2010PHYS\u2010N.A.\u2010MG\u2010TypeB\u2010FY23"]}],"id":[{"id":"10.13039\/100019465","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nanoparticles (NPs) supplementation to biodigesters improves the digestibility of biowaste and the generation of biogas. This study investigates the impact of innovative nanoadditives on the microbiome of biodigesters. Fresh cow manure was anaerobically incubated in a water bath under mesophilic conditions for 30\u2009days. Three different NPs (zinc ferrite, zinc ferrite with 10% carbon nanotubes and zinc ferrite with 10% C76 fullerene) were separately supplemented to the biodigesters at the beginning of the incubation period. Methane and hydrogen production were monitored daily. Manure samples were collected from the digesters at different time points and the microbial communities inside the biodigesters were investigated via real\u2010time PCR and 16\u2009S rRNA gene amplicon\u2010sequencing. The results indicate that zinc ferrite NPs enhanced biogas production the most. The microbial community was significantly affected by NPs addition in terms of archaeal and bacterial 16\u2009S rRNAgene copy numbers. The three ZF formulations NPs augmented the abundance of members within the hydrogenotrophic methanogenic phyla Methanobacteriaceae. While Methanomassiliicoccacaea were enriched in ZF\/C76 supplemented biodigester due to a significant increase in hydrogen partial pressure, probably caused by the enrichment of Spirochaetaceae (genus\n                    <jats:italic>Treponema<\/jats:italic>\n                    ). Overall, NPs supplementation significantly enriched acetate\u2010producing members within Hungateiclostridiaceae in ZF\/CNTs, Dysgonomonadaceae in ZF and Spirochaetaceae ZF\/C76 biodigesters.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14173","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T02:09:29Z","timestamp":1669169369000},"page":"128-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Impact of innovative nanoadditives on biodigesters microbiome"],"prefix":"10.1111","volume":"16","author":[{"given":"Fatma Y.","family":"Hassaneen","sequence":"first","affiliation":[{"name":"Energy Materials Laboratory, Physics Department, School of Sciences and Engineering The American University in Cairo  New Cairo Egypt"},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy Assiut University  Assiut Egypt"},{"name":"Biology department, School of Sciences and Engineering The American University in Cairo  New Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rehab Z.","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Biology department, School of Sciences and Engineering The American University in Cairo  New Cairo Egypt"},{"name":"Max Planck institute for Terrestrial Microbiology  Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammed S.","family":"Abdallah","sequence":"additional","affiliation":[{"name":"Energy Materials Laboratory, Physics Department, School of Sciences and Engineering The American University in Cairo  New Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nashaat","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Energy Materials Laboratory, Physics Department, School of Sciences and Engineering The American University in Cairo  New Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shereen M. 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