{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:56:21Z","timestamp":1783817781823,"version":"3.55.0"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T00:00:00Z","timestamp":1671494400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Yarrowia lipolytica<\/jats:italic>\n                    is a dimorphic oleaginous non\u2010conventional yeast widely used as a powerful host for expressing heterologous proteins, as well as a promising source of engineered cell factories for various applications. This microorganism has a documented use in Feed and Food and a GRAS (generally recognized as safe) status. Moreover, in vivo studies demonstrated a beneficial effect of this yeast on animal health. However, despite the focus on\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    for the industrial manufacturing of heterologous proteins and for probiotic effects, its potential for oral delivery of recombinant therapeutic proteins has seldom been evaluated in mammals. As the first steps towards this aim, we engineered two\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    strains, a dairy strain and a laboratory strain, to produce the model fluorescent protein mCherry. We demonstrated that both\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    strains transiently persisted for at least 1\u2009week after four daily oral administrations and they maintained the active expression of mCherry in the mouse intestine. We used confocal microscopy to image individual\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    cells of freshly collected intestinal tissues. They were found essentially in the lumen and they were rarely in contact with epithelial cells while transiting through the ileum, caecum and colon of mice. Taken as a whole, our results have shown that fluorescent\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    strains constitute novel tools to study the persistence and dynamics of orally administered yeasts which could be used in the future as oral delivery vectors for the secretion of active therapeutic proteins in the gut.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14178","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T02:59:22Z","timestamp":1671591562000},"page":"618-631","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Study of the persistence and dynamics of recombinant\n                    <scp>mCherry<\/scp>\n                    \u2010producing\n                    <i>Yarrowia lipolytica<\/i>\n                    strains in the mouse intestine using fluorescence imaging"],"prefix":"10.1111","volume":"16","author":[{"given":"Catherine","family":"Madzak","sequence":"first","affiliation":[{"name":"INRAE, AgroParisTech, Paris\u2010Saclay University, UMR 782 SayFood  Thiverval\u2010Grignon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Poiret","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 9017 \u2013 Center for Infection and Immunity of Lille  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Salom\u00e9 Desnoulez","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, US 41 \u2010 UMS 2014 \u2013 PLBS  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Folign\u00e9","sequence":"additional","affiliation":[{"name":"Univ. Lille, INSERM, CHU Lille, U1286 \u2010 Infinite \u2010 Institute for Translational Research in Inflammation  Lille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Lafont","sequence":"additional","affiliation":[{"name":"Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 9017 \u2013 Center for Infection and Immunity of Lille  Lille France"},{"name":"Univ. 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