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The glucagon\u2010like peptide\u20101 receptor (GLP\u20101R) agonists have shown great potential in treating diabetes mellitus, neurodegenerative diseases, obesity and hypertension. Butyric acid is a potential target in treating hypertension. Yet, the application of GLP\u20101 analogue and butyric acid in reducing blood pressure and reversing ventricular hypertrophy remains untapped. In this study, we combined the therapeutic capability of GLP\u20101 and butyric acid by transforming\n                    <jats:italic>Clostridium butyricum<\/jats:italic>\n                    (CB) with recombinant plasmid pMTL007 encoded with hGLP gene to construct the engineered probiotics\n                    <jats:italic>Clostridium butyricum<\/jats:italic>\n                    \u2010pMTL007\u2010GLP\u20101 (CB\u2010GLP\u20101). We used spontaneous hypertensive rat (SHR) models to evaluate the positive effect of this strain in treating hypertension. The results revealed that the intragastric administration of CB\u2010GLP\u20101 had markedly reduced blood pressure and improved cardiac marker ACE2, AT2R, AT1R, ANP, BNP, \u03b2\u2010MHC, \u03b1\u2010SMA and activating AMPK\/mTOR\/p70S6K\/4EBP1\u00a0signalling pathway. The high\u2010throughput sequencing further demonstrated that CB\u2010GLP\u20101 treatments significantly improved the dysbiosis in the SHR rats via downregulating the relative abundance of Porphyromonadaceae at the family level and upregulating\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    at the genus level. Hence, we concluded that the CB\u2010GLP\u20101 greatly improves blood pressure and cardiomegaly by restoring the gut microbiome and reducing ventricular hypertrophy in rat models. This is the first time using engineered CB in treating hypertension, which provides a new idea for the clinical treatment of hypertension.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14196","type":"journal-article","created":{"date-parts":[[2022,12,18]],"date-time":"2022-12-18T14:31:10Z","timestamp":1671373870000},"page":"799-812","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Engineered probiotics Clostridium butyricum\n                    <scp>\u2010pMTL007\u2010GLP<\/scp>\n                    \u20101 improves blood pressure via producing\n                    <scp>GLP<\/scp>\n                    \u20101 and modulating gut microbiota in spontaneous hypertension rat models"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7339-3878","authenticated-orcid":false,"given":"Xin\u2010liang","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Poyang Lake Environment and Resource Utilization, School of Resources Environmental and Chemical Engineering Ministry of Education, Nanchang University  Nanchang China"},{"name":"National Engineering Research Center for Bioengineering Drugs and Technologies Institute of Translational Medicine, Nanchang University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0078-3805","authenticated-orcid":false,"given":"Wen\u2010jie","family":"Chen","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Bioengineering Drugs and Technologies Institute of Translational Medicine, Nanchang University  Nanchang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Jin","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Bioengineering Drugs and Technologies Institute of Translational Medicine, Nanchang University  Nanchang 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