{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:29:15Z","timestamp":1775672955554,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2021J011279"],"award-info":[{"award-number":["2021J011279"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Currently, malaria is still one of the major public health problems commonly caused by the four\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species. The similar symptoms of malaria and the COVID\u201019 epidemic of fever or fatigue lead to frequent misdiagnosis. The disadvantages of existing detection methods, such as time\u2010consuming, costly, complicated operation, need for experienced technicians, and indistinguishable typing, lead to difficulties in meeting the clinical requirements of rapid, easy, and accurate typing of common\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species. In this study, we developed and optimized a universal two\u2010dimensional labelled probe\u2010mediated melting curve analysis (UP\u2010MCA) assay based on multiplex and asymmetric PCR for rapid and accurate typing of five\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species, including novel human\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    ,\n                    <jats:italic>Plasmodium knowlesi<\/jats:italic>\n                    (Pk), in a single closed tube following genome extraction. The assay showed a limit of detection (LOD) of 10 copies per reaction and could accurately distinguish\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species from intra\u2010plasmodium and other pathogens. Additionally, we proposed and validated different methods of fluorescence quenching and tag design for probes that are suitable for UP\u2010MCA assays. Moreover, the clinical performance of the\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    UP\u2010MCA assay using a base\u2010quenched universal probe was evaluated using 226 samples and showed a sensitivity of 100% (164\/164) and specificity of 100% (62\/62) at a 99% confidence interval, with the microscopy method as the gold standard. In summary, the UP\u2010MCA assay showed excellent sensitivity, specificity, and accuracy for genotyping\n                    <jats:italic>Plasmodium\u00a0species<\/jats:italic>\n                    spp. Additionally, it facilitates convenient and rapid\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    detection in routine clinical practice and has great potential for clinical translation.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14232","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T13:39:28Z","timestamp":1675690768000},"page":"838-846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Universal two\u2010dimensional labelled probe\u2010mediated melting curve analysis based on multiplex\n                    <scp>PCR<\/scp>\n                    for rapid typing of\n                    <i>Plasmodium<\/i>\n                    in a single closed tube"],"prefix":"10.1111","volume":"16","author":[{"given":"Haipo","family":"Xu","sequence":"first","affiliation":[{"name":"College of Chemical Engineering, Fuzhou University  Fuzhou China"},{"name":"The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University  Fuzhou China"},{"name":"Mengchao Med\u2010X Center Fuzhou University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yupeng","family":"Sun","sequence":"additional","affiliation":[{"name":"The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University  Fuzhou China"},{"name":"Mengchao Med\u2010X Center Fuzhou University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolong","family":"Zhang","sequence":"additional","affiliation":[{"name":"The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University  Fuzhou China"},{"name":"Mengchao Med\u2010X Center Fuzhou University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronghua","family":"Chen","sequence":"additional","affiliation":[{"name":"The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixiong","family":"Cai","sequence":"additional","affiliation":[{"name":"The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University  Fuzhou China"},{"name":"Mengchao Med\u2010X Center Fuzhou University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bixing","family":"Zhao","sequence":"additional","affiliation":[{"name":"The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University  Fuzhou China"},{"name":"Mengchao Med\u2010X Center Fuzhou University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3096-4981","authenticated-orcid":false,"given":"Xiaolong","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering, Fuzhou University  Fuzhou China"},{"name":"The United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province Mengchao Hepatobiliary Hospital of Fujian Medical University  Fuzhou China"},{"name":"Mengchao Med\u2010X Center Fuzhou University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering, Fuzhou University  Fuzhou China"},{"name":"Fujian Cancer Hospital &amp; Fujian Medical University Cancer Hospital  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03285-12"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-12-405"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2020.102069"},{"key":"e_1_2_8_5_1","volume-title":"Guidelines for the treatment of malaria","author":"Global Maiaria Programme, World Health Organization","year":"2015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2007.10.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2381-7"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191886"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2020.105511"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.17-0738"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01879-19"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-015-0542-5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2010196117"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.594808"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-021-03753-8"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03379-2"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-016-1676-9"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03507.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00958-13"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.12.5636-5643.2004"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00079-12"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15836-4"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2399-x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1086\/524889"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis228"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b02030"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14232","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.14232","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:00:55Z","timestamp":1762272055000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.14232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,6]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["10.1111\/1751-7915.14232"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.14232","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2022.04.12.22271963","asserted-by":"object"}]},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,6]]},"assertion":[{"value":"2022-04-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-01-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}