{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:23:59Z","timestamp":1789698239444,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,7,25]],"date-time":"2023-07-25T00:00:00Z","timestamp":1690243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009097","name":"Fondazione Cariparma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009097","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The lower female reproductive tract is notoriously dominated by\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    species, among which\n                    <jats:italic>Lactobacillus crispatus<\/jats:italic>\n                    emerges for its protective and health\u2010promoting activities. Although previous comparative genome analyses highlighted genetic and phenotypic diversity within the\n                    <jats:italic>L.\u2009crispatus<\/jats:italic>\n                    species, most studies have focused on the presence\/absence of accessory genes. Here, we investigated the variation at the single nucleotide level within protein\u2010encoding genes shared across a human\u2010derived\n                    <jats:italic>L.\u2009crispatus<\/jats:italic>\n                    strain selection, which includes 200 currently available human\u2010derived\n                    <jats:italic>L.\u2009crispatus<\/jats:italic>\n                    genomes as well as 41 chromosome sequences of such taxon that have been decoded in the framework of this study. Such data clearly pointed out the presence of intra\u2010species micro\u2010diversities that could have evolutionary significance contributing to phenotypical diversification by affecting protein domains. Specifically, two single nucleotide variations in the type II pullulanase gene sequence led to specific amino acid substitutions, possibly explaining the substantial differences in the growth performances and competition abilities observed in a multi\u2010strain bioreactor culture simulating the vaginal environment. Accordingly,\n                    <jats:italic>L.\u2009crispatus<\/jats:italic>\n                    strains display different growth performances, suggesting that the colonisation and stable persistence in the female reproductive tract between the members of this taxon is highly variable.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14305","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T01:16:12Z","timestamp":1690334172000},"page":"1774-1789","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The core genome evolution of\n                    <i>Lactobacillus crispatus<\/i>\n                    as a driving force for niche competition in the human vaginal tract"],"prefix":"10.1111","volume":"16","author":[{"given":"Chiara","family":"Tarracchini","sequence":"first","affiliation":[{"name":"Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Argentini","sequence":"additional","affiliation":[{"name":"Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulia","family":"Alessandri","sequence":"additional","affiliation":[{"name":"Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability University of Parma  Parma Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3024-0537","authenticated-orcid":false,"given":"Gabriele Andrea","family":"Lugli","sequence":"additional","affiliation":[{"name":"Laboratory of Probiogenomics, Department of Chemistry, Life Sciences, and Environmental Sustainability University of Parma  Parma 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