{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:10:53Z","timestamp":1790205053796,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia","doi-asserted-by":"publisher","award":["2020MS08046"],"award-info":[{"award-number":["2020MS08046"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human infections caused by\n                    <jats:italic>Brucella<\/jats:italic>\n                    (called brucellosis) are among the most common zoonoses worldwide with an estimated 500,000 cases each year. Since chronic\n                    <jats:italic>Brucella<\/jats:italic>\n                    infections are extremely difficult to treat, there is an urgent need for more effective therapeutics. As a facultative intracellular bacterium,\n                    <jats:italic>Brucella<\/jats:italic>\n                    is strictly parasitic in the host cell. Here, we performed proteomic and transcriptomic and metabolomic analyses on\n                    <jats:italic>Brucella<\/jats:italic>\n                    infected patients, mice and cells that provided an extensive \u201cmap\u201d of physiological changes in brucellosis patients and characterized the metabolic pathways essential to the response to infection, as well as the associated cellular response and molecular mechanisms. This is the first report utilizing multi\u2010omics analysis to investigate the global response of proteins and metabolites associated with\n                    <jats:italic>Brucella<\/jats:italic>\n                    infection, and the data can provide a comprehensive insight to understand the mechanism of\n                    <jats:italic>Brucella<\/jats:italic>\n                    infection. We demonstrated that\n                    <jats:italic>Brucella<\/jats:italic>\n                    increased nucleotide synthesis in the host, consistent with increased biomass requirement. We also identified IMPDH2, a key regulatory complex that controls nucleotide synthesis during\n                    <jats:italic>Brucella<\/jats:italic>\n                    infection. Pharmacological targeting of IMPDH2, the rate\u2010limiting enzyme in guanine nucleotide biosynthesis, efficiently inhibits\n                    <jats:italic>B.\u2009abortus<\/jats:italic>\n                    growth both in vitro and in vivo. Through screening a library of natural products, we identified oxymatrine, an alkaloid obtained primarily from Sophora roots, is a novel and selective IMPDH2 inhibitor. In further in vitro bacterial inhibition assays, oxymatrine effectively inhibited the growth of\n                    <jats:italic>B.\u2009abortus<\/jats:italic>\n                    , which was impaired by exogenous supplementation of guanosine, a salvage pathway of purine nucleotides. This moderately potent, structurally novel compound may provide clues for further design and development of efficient IMPDH2 inhibitors and also demonstrates the potential of natural compounds from plants against\n                    <jats:italic>Brucella<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14307","type":"journal-article","created":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T08:21:10Z","timestamp":1698308470000},"page":"2345-2366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Combining multi\u2010omics analysis to identify host\u2010targeted targets for the control of\n                    <i>Brucella<\/i>\n                    infection"],"prefix":"10.1111","volume":"16","author":[{"given":"Jiuwang","family":"Yu","sequence":"first","affiliation":[{"name":"TCM Hospital of Mongolian Medicine in Hohhot  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Pathology Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarong","family":"Guo","sequence":"additional","affiliation":[{"name":"TCM Hospital of Mongolian Medicine in Hohhot  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiheng","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sha","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bilige","family":"Aode","sequence":"additional","affiliation":[{"name":"Department of Mongolian Medicine Inner Mongolia Xilin Gol League Mongolian Medical Hospital  Xilinhaote China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachula","family":"Baoyin","sequence":"additional","affiliation":[{"name":"Mongolia Medical School Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8485-3566","authenticated-orcid":false,"given":"Lidao","family":"Bao","sequence":"additional","affiliation":[{"name":"TCM Hospital of Mongolian Medicine in Hohhot  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Wu","sequence":"additional","affiliation":[{"name":"TCM Hospital of Mongolian Medicine in Hohhot  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/dta.1798"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2020.1726342"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02789"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2020.05.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.09.007"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2789"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-018-0980-3"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0193749"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9100844"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9121040"},{"key":"e_1_2_10_12_1","first-page":"1913","article-title":"Effects of mycophenolate mofetil on proliferation and mucin\u20105AC expression in human conjunctival goblet cells in vitro","volume":"16","author":"He H.","year":"2010","journal-title":"Molecular Vision"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0209184"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092099"},{"issue":"9","key":"e_1_2_10_15_1","article-title":"Genome\u2010wide analysis of the PIN auxin efflux carrier gene family in coffee","volume":"9","author":"Huang X.","year":"2020","journal-title":"Plants (Basel, Switzerland)"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12990"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/354649"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53243"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-017-1794-y"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","unstructured":"Li S. Feng G. Zhang M. Zhang X. Lu J. Feng C.et al. (2022)Oxymatrine attenuates TNBS\u2010induced colinutis in rats through TLR9\/Myd88\/NF\u2010\u03baB signal pathway 41 9603271221078866.","DOI":"10.1177\/09603271221078866"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00105"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0186484"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi300511w"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971-4065.91188"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00298"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-06654-y"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19486-2"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.56418"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2020.04.007"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.04.002"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E18-05-0274"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.7b00249"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021978"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b11575"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.07.009"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00887-1"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-020-00720-4"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:24:29Z","timestamp":1762273469000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.14307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,26]]},"references-count":36,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1111\/1751-7915.14307"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.14307","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,26]]},"assertion":[{"value":"2022-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}