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From an industrial biotechnology perspective, extending the acyl\u2010CoA portfolio of a cell factory is vital to producing industrial relevant odd\u2010chain alcohols, acids, ketones and polyketides. The bioproduction of odd\u2010chain molecules can be facilitated by incorporating propionyl\u2010CoA into the metabolic network. The shortest pathway for propionyl\u2010CoA production, which relies on succinyl\u2010CoA catabolism encoded by the sleeping beauty mutase operon, was evaluated in\n                    <jats:italic>Pseudomonas taiwanensis<\/jats:italic>\n                    VLB120. A single genomic copy of the sleeping beauty mutase genes\n                    <jats:italic>scpA<\/jats:italic>\n                    ,\n                    <jats:italic>argK<\/jats:italic>\n                    and\n                    <jats:italic>scpB<\/jats:italic>\n                    combined with the deletion of the methylcitrate synthase PVLB_08385 was sufficient to observe propionyl\u2010CoA accumulation in this\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    . The chassis' capability for odd\u2010chain product synthesis was assessed by expressing an acyl\u2010CoA hydrolase, which enabled propionate synthesis. Three fed\u2010batch strategies during bioreactor fermentations were benchmarked for propionate production, in which a maximal propionate titre of 2.8\u2009g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    was achieved. Considering that the fermentations were carried out in mineral salt medium under aerobic conditions and that a single genome copy drove propionyl\u2010CoA production, this result highlights the potential of\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    to produce propionyl\u2010CoA derived, odd\u2010chain products.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14309","type":"journal-article","created":{"date-parts":[[2023,8,4]],"date-time":"2023-08-04T00:47:01Z","timestamp":1691110021000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expanding\n                    <b>\n                      <i>Pseudomonas taiwanensis<\/i>\n                    <\/b>\n                    VLB120's acyl\u2010CoA portfolio: Propionate production in mineral salt 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