{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T17:43:24Z","timestamp":1792086204133,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T00:00:00Z","timestamp":1691539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32002305"],"award-info":[{"award-number":["32002305"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2022A1515011104"],"award-info":[{"award-number":["2022A1515011104"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    is a ubiquitous pathogen that infects all warm\u2010blooded animals, including humans, causing substantial socioeconomic and healthcare burdens. However, there is no ideal vaccine for toxoplasmosis. As metabolism is important in the growth and virulence of\n                    <jats:italic>Toxoplasma<\/jats:italic>\n                    , some key pathways are promising antiparasitic targets. Here, we identified 6\u2010phosphogluconate dehydrogenase 1 (\n                    <jats:italic>Tg<\/jats:italic>\n                    6PGDH1) in the oxidative pentose phosphate pathway as a cytoplasmic protein that is dispensable for tachyzoite growth of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    in vitro but critical for virulence and cyst formation in vivo. The depletion of\n                    <jats:italic>Tg<\/jats:italic>\n                    6PGDH1 causes decreased gene transcription involved in signal transduction, transcriptional regulation and virulence. Furthermore, we analysed the protective effect of the ME49\n                    <jats:italic>\u03946pgdh1<\/jats:italic>\n                    mutant as an attenuated vaccine and found that ME49\n                    <jats:italic>\u03946pgdh1<\/jats:italic>\n                    immunization stimulated strong protective immunity against lethal challenges and blocked cyst formation caused by reinfection. Furthermore, we showed that ME49\n                    <jats:italic>\u03946pgdh1<\/jats:italic>\n                    immunization stimulated increased levels of interferon\u2010gamma, tumour necrosis factor\u2010alpha and\n                    <jats:italic>Toxoplasma\u2010<\/jats:italic>\n                    specific IgG antibodies. These data highlight the role of\n                    <jats:italic>Tg<\/jats:italic>\n                    6PGDH1 in the growth and virulence of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    and its potential as a target for the development of a live\u2010attenuated vaccine.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14324","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T11:35:16Z","timestamp":1691580916000},"page":"1957-1970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Role of 6\u2010phosphogluconate dehydrogenase enzyme 1 in growth and virulence of\n                    <i>Toxoplasma gondii<\/i>\n                    and development of attenuated live vaccine"],"prefix":"10.1111","volume":"16","author":[{"given":"Qinghong","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefang","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuo","family":"Ji","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bang","family":"Shen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Zhong","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, College of Materials and Energy South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyu","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention South China Agricultural University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6090-7400","authenticated-orcid":false,"given":"Ningbo","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention South China Agricultural University  Guangzhou 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