{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:05:29Z","timestamp":1791929129342,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:00:00Z","timestamp":1692576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    High quinolone resistance of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    limits the therapy options for urinary tract infection (UTI). In response to the urgent need for efficient treatment of multidrug\u2010resistant infections, we designed a fimbriae targeting superparamagnetic iron oxide nanoparticle (SPION) delivering ciprofloxacin to ciprofloxacin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Bovine serum albumin (BSA) conjugated poly(acrylic acid) (PAA) coated SPIONs (BSA@PAA@SPION) were developed for encapsulation of ciprofloxacin and the nanoparticles were tagged with 4\u2010aminophenyl\u2010\u03b1\u2010D\u2010mannopyrannoside (mannoside, Man) to target\n                    <jats:italic>E. coli<\/jats:italic>\n                    fimbriae. Ciprofloxacin\u2010loaded mannoside tagged nanoparticles (Cip\u2010Man\u2010BSA@PAA@SPION) provided high antibacterial activity (97.1 and 97.5%, respectively) with a dose of 32\u2009\u03bcg\/mL ciprofloxacin against two ciprofloxacin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates. Furthermore, a strong biofilm inhibition (86.9% and 98.5%, respectively) was achieved in the isolates at a dose 16 and 8 times lower than the minimum biofilm eradication concentration (MBEC) of ciprofloxacin. Weaker growth inhibition was observed with untargeted nanoparticles, Cip\u2010BSA@PAA@SPIONs, confirming that targeting\n                    <jats:italic>E. coli<\/jats:italic>\n                    fimbria with mannoside\u2010tagged nanoparticles increases the ciprofloxacin efficiency to treat ciprofloxacin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Enhanced killing activity against ciprofloxacin\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    planktonic cells and strong growth inhibition of their biofilms suggest that Cip\u2010Man\u2010BSA@PAA@SPION system might be an alternative and\/or complementary therapeutic option for the treatment of quinolone\u2010resistant\n                    <jats:italic>E. coli<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14327","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T22:18:13Z","timestamp":1692656293000},"page":"2072-2081","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Fimbria targeting superparamagnetic iron oxide nanoparticles enhance the antimicrobial and antibiofilm activity of ciprofloxacin against quinolone\u2010resistant\n                    <i>E. coli<\/i>"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7110-1859","authenticated-orcid":false,"given":"Nazli","family":"Atac","sequence":"first","affiliation":[{"name":"School of Medicine, Medical Microbiology Ko\u00e7 University  Istanbul Turkey"},{"name":"Ko\u00e7 University\u2010\u0130\u015fbank Center for Infectious Diseases (KUISCID)  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kubra","family":"Onbasli","sequence":"additional","affiliation":[{"name":"Department of Metallurgical and Materials Engineering \u0130stanbul Technical University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irem","family":"Koc","sequence":"additional","affiliation":[{"name":"Graduate School of Materials Science and Engineering Ko\u00e7 University  Istanbul 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