{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:54:01Z","timestamp":1789095241375,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,7]],"date-time":"2024-03-07T00:00:00Z","timestamp":1709769600000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["FSC 2186"],"award-info":[{"award-number":["FSC 2186"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Plastic usage by microbes as a carbon source is a promising strategy to increase the recycling quota. 1,4\u2010butanediol (BDO) is a common monomer derived from polyesters and polyurethanes. In this study,\n                    <jats:italic>Ustilago trichophora<\/jats:italic>\n                    was found to be an efficient cell\u2010factory to valorize BDO. To investigate product formation by\n                    <jats:italic>U.\u2009trichophora<\/jats:italic>\n                    , we refined the traditional ion exclusion liquid chromatography method by examining eluent, eluent concentrations, oven temperatures, and organic modifiers to make the chromatography compatible with mass spectrometry. An LC\u2010UV\/RI\u2010MS\n                    <jats:sup>2<\/jats:sup>\n                    method is presented here to identify and quantify extracellular metabolites in the cell cultures. With this method, we successfully identified that\n                    <jats:italic>U.\u2009trichophora<\/jats:italic>\n                    secreted malic acid, succinic acid, erythritol, and mannitol into the culture medium. Adaptive laboratory evolution followed by medium optimization significantly improved\n                    <jats:italic>U.\u2009trichophora<\/jats:italic>\n                    growth on BDO and especially malic acid production. Overall, the carbon yield on the BDO substrate was approximately 33% malic acid. This study marks the first report of a Ustilaginaceae fungus capable of converting BDO into versatile chemical building blocks. Since\n                    <jats:italic>U.\u2009trichophora<\/jats:italic>\n                    is not genetically engineered, it is a promising microbial host to produce malic acid from BDO, thereby contributing to the development of the envisaged sustainable bioeconomy.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14384","type":"journal-article","created":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T00:29:54Z","timestamp":1709857794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Unlocking the potentials of\n                    <i>Ustilago trichophora<\/i>\n                    for up\u2010cycling polyurethane\u2010derived monomer 1,4\u2010butanediol"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1438-416X","authenticated-orcid":false,"given":"An N. 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