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Yet, existing methods are complex and costly. Biosensors offer an alternative. While many biosensors detect various antibiotics, specific ones for beta\u2010lactams are lacking. To address this gap, a biosensor based on the AmpC beta\u2010lactamase regulation system (\n                    <jats:italic>ampR\u2013ampC<\/jats:italic>\n                    ) from\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    sp. IB20, an Antarctic isolate, was developed in this study. The AmpR\u2013AmpC system is well\u2010conserved in the genus\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    and has been extensively studied for its involvement in peptidoglycan recycling and beta\u2010lactam resistance. To create the biosensor, the\n                    <jats:italic>ampC<\/jats:italic>\n                    coding sequence was replaced with the mCherry fluorescent protein as a reporter, resulting in a transcriptional fusion. This construct was then inserted into\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    SN0301, a beta\u2010lactam hypersensitive strain, generating a whole\u2010cell biosensor. The biosensor demonstrated dose\u2010dependent detection of penicillins, cephalosporins and carbapenems. However, the most interesting aspect of this work is the high sensitivity presented by the biosensor in the detection of carbapenems, as it was able to detect 8\u2009pg\/mL of meropenem and 40\u2009pg\/mL of imipenem and reach levels of 1\u201310\u2009ng\/mL for penicillins and cephalosporins. This makes the biosensor a powerful tool for the detection of beta\u2010lactam antibiotics, specifically carbapenems, in different matrices.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14385","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T08:00:59Z","timestamp":1704873659000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["A whole\u2010cell hypersensitive biosensor for beta\u2010lactams based on the AmpR\u2010AmpC regulatory circuit from the Antarctic\n                    <i>Pseudomonas<\/i>\n                    sp. IB20"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0181-2846","authenticated-orcid":false,"given":"Sebasti\u00e1n","family":"Higuera\u2010Llant\u00e9n","sequence":"first","affiliation":[{"name":"Grupo de Resistencia Antimicrobiana en Bacterias Pat\u00f3genas y Ambientales, GRABPA, Instituto de Biolog\u00eda Pontificia Universidad Cat\u00f3lica de Valpara\u00edso  Valpara\u00edso Chile"},{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB\u2010R)  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1987-4600","authenticated-orcid":false,"given":"Manuel","family":"Alcalde\u2010Rico","sequence":"additional","affiliation":[{"name":"Grupo de Resistencia Antimicrobiana en Bacterias Pat\u00f3genas y Ambientales, GRABPA, Instituto de Biolog\u00eda Pontificia Universidad Cat\u00f3lica de Valpara\u00edso  Valpara\u00edso Chile"},{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB\u2010R)  Valpara\u00edso Chile"},{"name":"Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen Macarena, CSIC, Universidad de Sevilla  Sevilla Spain"},{"name":"CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9447-8325","authenticated-orcid":false,"given":"Felipe","family":"Vasquez\u2010Ponce","sequence":"additional","affiliation":[{"name":"Grupo de Resistencia Antimicrobiana en Bacterias Pat\u00f3genas y Ambientales, GRABPA, Instituto de Biolog\u00eda Pontificia Universidad Cat\u00f3lica de Valpara\u00edso  Valpara\u00edso Chile"},{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB\u2010R)  Valpara\u00edso Chile"},{"name":"Department of Microbiology, Institute of Biomedical Sciences Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1978-6109","authenticated-orcid":false,"given":"Claudia","family":"Ibacache\u2010Quiroga","sequence":"additional","affiliation":[{"name":"Escuela de Nutrici\u00f3n y Diet\u00e9tica, Facultad de Farmacia Universidad de Valpara\u00edso  Valpara\u00edso Chile"},{"name":"Centro de Micro\u2010Bioinnovaci\u00f3n Universidad de Valpara\u00edso  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0495-3848","authenticated-orcid":false,"given":"Jes\u00fas","family":"Blazquez","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology, Consejo Superior de Investigaciones Cient\u00edficas (CSIC)  Madrid 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