{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:39:49Z","timestamp":1791812389704,"version":"4.3.4"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T00:00:00Z","timestamp":1714348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Soluble di\u2010iron monooxygenases (SDIMOs) are multi\u2010component enzymes catalysing the oxidation of various substrates. These enzymes are characterized by high sequence and functional diversity that is still not well understood despite their key role in biotechnological processes including contaminant biodegradation. In this study, we analysed a mutant of\n                    <jats:italic>Rhodoccocus aetherivorans<\/jats:italic>\n                    BCP1 (BCP1\u20102.10) characterized by a transposon insertion in the gene\n                    <jats:italic>smoA<\/jats:italic>\n                    encoding the alpha subunit of the plasmid\u2010located SDIMO SmoABCD. The mutant BCP1\u20102.10 showed a reduced capacity to grow on propane, lost the ability to grow on butane, pentane and\n                    <jats:italic>n\u2010<\/jats:italic>\n                    hexane and was heavily impaired in the capacity to degrade chloroform and trichloroethane. The expression of the additional SDIMO\n                    <jats:italic>prmABCD<\/jats:italic>\n                    in BCP1\u20102.10 probably allowed the mutant to partially grow on propane and to degrade it, to some extent, together with the other short\u2010chain\n                    <jats:italic>n\u2010<\/jats:italic>\n                    alkanes. The complementation of the mutant, conducted by introducing\n                    <jats:italic>smoABCD<\/jats:italic>\n                    in the genome as a single copy under a constitutive promoter or within a plasmid under a thiostreptone\u2010inducible promoter, allowed the recovery of the alkanotrophic phenotype as well as the capacity to degrade chlorinated\n                    <jats:italic>n<\/jats:italic>\n                    \u2010alkanes. The heterologous expression of\n                    <jats:italic>smoABCD<\/jats:italic>\n                    allowed a non\u2010alkanotrophic\n                    <jats:italic>Rhodococcus<\/jats:italic>\n                    strain to grow on pentane and\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane when the gene cluster was introduced together with the downstream genes encoding alcohol and aldehyde dehydrogenases and a GroEL chaperon. BCP1\n                    <jats:italic>smoA<\/jats:italic>\n                    gene was shown to belong to the group 6 SDIMOs, which is a rare group of monooxygenases mostly present in\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    genus and in a few\n                    <jats:italic>Rhodococcus<\/jats:italic>\n                    strains. SmoABCD originally evolved in\n                    <jats:italic>Mycobacterium<\/jats:italic>\n                    and was then acquired by\n                    <jats:italic>Rhodococcus<\/jats:italic>\n                    through horizontal gene transfer events. This work extends the knowledge of the biotechnologically relevant SDIMOs by providing functional and evolutionary insights into a group 6 SDIMO in\n                    <jats:italic>Rhodococcus<\/jats:italic>\n                    and demonstrating its key role in the metabolism of short\u2010chain alkanes and degradation of chlorinated\n                    <jats:italic>n<\/jats:italic>\n                    \u2010alkanes.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14453","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T11:54:15Z","timestamp":1714391655000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Unravelling the role of the group 6 soluble di\u2010iron monooxygenase (\n                    <scp>SDIMO<\/scp>\n                    )\n                    <scp>SmoABCD<\/scp>\n                    in alkane metabolism and chlorinated alkane degradation"],"prefix":"10.1111","volume":"17","author":[{"given":"Eleonora","family":"Ferrari","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Biotechnology (FaBit) University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Di\u00a0Benedetto","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology (FaBit) University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Firrincieli","sequence":"additional","affiliation":[{"name":"Department for Innovation in Biological, Agro\u2010Food and Forest Systems University of Tuscia  Viterbo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Presentato","sequence":"additional","affiliation":[{"name":"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF) University of Palermo  Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Frascari","sequence":"additional","affiliation":[{"name":"Department of Civil, Chemical, Environmental and Materials Engineering (DICAM) University of Bologna  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6238-8296","authenticated-orcid":false,"given":"Martina","family":"Cappelletti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Biotechnology (FaBit) University of Bologna  Bologna 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