{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:44:41Z","timestamp":1790790281347,"version":"4.1.0"},"reference-count":81,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T00:00:00Z","timestamp":1718582400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["757563"],"award-info":[{"award-number":["757563"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["45809066"],"award-info":[{"award-number":["45809066"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Pathogens resistant to classical control strategies pose a significant threat to crop yield, with seeds being a major transmission route. Bacteriophages, viruses targeting bacteria, offer an environmentally sustainable biocontrol solution. In this study, we isolated and characterized two novel phages, Athelas and Alfirin, which infect\n                    <jats:italic>Pseudomonas syringae<\/jats:italic>\n                    and\n                    <jats:italic>Agrobacterium fabrum<\/jats:italic>\n                    , respectively, and included the recently published Pfeifenkraut phage infecting\n                    <jats:italic>Xanthomonas translucens<\/jats:italic>\n                    . Using a simple immersion method, phages coated onto seeds successfully lysed bacteria post air\u2010drying. The seed coat mucilage (SCM), a polysaccharide\u2013polymer matrix exuded by seeds, plays a critical role in phage binding. Seeds with removed mucilage formed five to 10 times less lysis zones compared to those with mucilage. The podovirus Athelas showed the highest mucilage dependency. Phages from the\n                    <jats:italic>Autographiviridae<\/jats:italic>\n                    family also depended on mucilage for seed adhesion. Comparative analysis of\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    SCM mutants suggested the diffusible cellulose as a key component for phage binding. Long\u2010term activity tests demonstrated high phage stability on seed surfaces and significantly increasing seedling survival rates in the presence of pathogens. Using non\u2010virulent host strains enhanced phage presence on seeds but also has potential limitations. These findings highlight phage\u2010based interventions as promising, sustainable strategies for combating pathogen resistance and improving crop yield.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14507","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T08:53:42Z","timestamp":1718614422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Seed coating with phages for sustainable plant biocontrol of plant pathogens and influence of the seed coat mucilage"],"prefix":"10.1111","volume":"17","author":[{"given":"Sebastian H.","family":"Erdrich","sequence":"first","affiliation":[{"name":"Forschungszentrum J\u00fclich Department for Plant Sciences (IBG\u20102), Institute of Bio\u2010 and Geosciences  J\u00fclich Germany"},{"name":"Forschungszentrum J\u00fclich Department for Biotechnology (IBG\u20101), Institute of Bio\u2010 and Geosciences  J\u00fclich 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