{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T06:40:30Z","timestamp":1780555230299,"version":"3.54.1"},"reference-count":59,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018833","name":"Agence de l'innovation de D\u00e9fense","doi-asserted-by":"publisher","award":["ANR\u201017\u2010ASTR\u20100018"],"award-info":[{"award-number":["ANR\u201017\u2010ASTR\u20100018"]}],"id":[{"id":"10.13039\/501100018833","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Chassis strains, derived from\n                    <jats:italic>Streptomyces coelicolor<\/jats:italic>\n                    M145, deleted for one or more of its four main specialized metabolites biosynthetic pathways (CPK, CDA, RED and ACT), in various combinations, were constructed for the heterologous expression of specialized metabolites biosynthetic pathways of various types and origins. To determine consequences of these deletions on the metabolism of the deleted strains comparative lipidomic and metabolomic analyses of these strains and of the original strain were carried out. These studies unexpectedly revealed that the deletion of the peptidic clusters, RED and\/or CDA, in a strain deleted for the ACT cluster, resulted into a great increase in the triacylglycerol (TAG) content, whereas the deletion of polyketide clusters, ACT and CPK had no impact on TAG content. Low or high TAG content of the deleted strains was correlated with abundance or paucity in amino acids, respectively, reflecting high or low activity of oxidative metabolism. Hypotheses based on what is known on the bio\u2010activity and the nature of the precursors of these specialized metabolites are proposed to explain the unexpected consequences of the deletion of these pathways on the metabolism of the bacteria and on the efficiency of the deleted strains as chassis strains.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.14538","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T11:25:47Z","timestamp":1722597947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Consequences of the deletion of the major specialized metabolite biosynthetic pathways of\n                    <i>Streptomyces coelicolor<\/i>\n                    on the metabolome and lipidome of this strain"],"prefix":"10.1111","volume":"17","author":[{"given":"Clara","family":"Lejeune","sequence":"first","affiliation":[{"name":"Institut de Biologie Int\u00e9grative de la Cellule (I2BC, UMR 9198), Universit\u00e9 Paris\u2010Saclay, CEA, CNRS, Group MES (M\u00e9tabolisme Energ\u00e9tique Des Streptomyces)  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Abreu","sequence":"additional","affiliation":[{"name":"UFR Pharmacie, Universit\u00e9 Paris\u2010Saclay, CNRS, Group \u00abLipides, Syst\u00e8mes Analytiques et Biologiques (Lip(Sys)<sup>2<\/sup>\u00bb  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Gu\u00e9rard","sequence":"additional","affiliation":[{"name":"Institut Des Sciences Des Plantes (IPS2, UMR 9213), Universit\u00e9 Paris\u2010Saclay, CNRS, Plateforme \u00abSPOmics\u2010M\u00e9tabolome\u00bb  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Askora","sequence":"additional","affiliation":[{"name":"Institut de Biologie Int\u00e9grative de la Cellule (I2BC, UMR 9198), Universit\u00e9 Paris\u2010Saclay, CEA, CNRS, Group MES (M\u00e9tabolisme Energ\u00e9tique Des Streptomyces)  Gif\u2010sur\u2010Yvette France"},{"name":"Department of Botany and Microbiology, Faculty of Science Zagazig University  Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"David","sequence":"additional","affiliation":[{"name":"Institut de Biologie Int\u00e9grative de la Cellule (I2BC, UMR 9198), Universit\u00e9 Paris\u2010Saclay, CEA, CNRS, Group MES (M\u00e9tabolisme Energ\u00e9tique Des Streptomyces)  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Chaminade","sequence":"additional","affiliation":[{"name":"UFR Pharmacie, Universit\u00e9 Paris\u2010Saclay, CNRS, Group \u00abLipides, Syst\u00e8mes Analytiques et Biologiques (Lip(Sys)<sup>2<\/sup>\u00bb  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertrand","family":"Gaki\u00e8re","sequence":"additional","affiliation":[{"name":"Institut Des Sciences Des Plantes (IPS2, UMR 9213), Universit\u00e9 Paris\u2010Saclay, CNRS, Plateforme \u00abSPOmics\u2010M\u00e9tabolome\u00bb  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9509-4115","authenticated-orcid":false,"given":"Marie\u2010Joelle","family":"Virolle","sequence":"additional","affiliation":[{"name":"Institut de Biologie Int\u00e9grative de la Cellule (I2BC, UMR 9198), Universit\u00e9 Paris\u2010Saclay, CEA, CNRS, Group MES (M\u00e9tabolisme Energ\u00e9tique Des Streptomyces)  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.3.675-680.1990"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12040944"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-002-1098-1"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1129009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02638-07"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1139\/m77-108"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-019-09975-w"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/417141a"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2005.1"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsml\/uqad020"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.02.016"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(85)90220-3"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1710304"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084151"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9060280"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jz402393a"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11061470"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-00259-9"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bip.21493"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2016.02.012"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.1503.03042"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-7915.2010.00219.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2sc20410j"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-136-4-713"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2014.10.004"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.812788"},{"key":"e_1_2_9_28_1","first-page":"3575","article-title":"CDA is a new chromosomally\u2010determined antibiotic from Streptomyces coelicolor A3(2)","volume":"129","author":"Hopwood D.A.","year":"1983","journal-title":"Journal of General Microbiology"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14445"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.03.010"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12101421"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.623919"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.13965"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.739569"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-023-02244-6"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.7b00163"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003702817709459"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00008-08"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2024.06.005"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2009.01.015"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-140-4-931"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-006-0176-7"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-12-111"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.mmi_17040737.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45164-5"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.175990"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00261-23"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101525"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01459.x"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9120847"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01742"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-015-0335-0"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2023.09.001"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015447"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9020083"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01864-14"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0335-4"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1134-8"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.3390\/app112411819"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2010.02.111"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.14538","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:03:25Z","timestamp":1762272205000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.14538"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":59,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/1751-7915.14538"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.14538","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"2024-01-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14538"}}