{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T07:27:10Z","timestamp":1783668430495,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,24]],"date-time":"2024-11-24T00:00:00Z","timestamp":1732406400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010676","name":"H2020 Societal Challenges","doi-asserted-by":"publisher","award":["101000794"],"award-info":[{"award-number":["101000794"]}],"id":[{"id":"10.13039\/100010676","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microbial biosurfactants have garnered significant interest from industry due to their lower toxicity, biodegradability, activity at lower concentrations and higher resistance compared to synthetic surfactants. The deep\u2010sea\n                    <jats:italic>Rhodococcus<\/jats:italic>\n                    sp. I2R has been identified as a producer of glycolipid biosurfactants, specifically succinoyl trehalolipids, which exhibit antiviral activity. However, genome mining of this bacterium has revealed a still unexplored repertoire of biosurfactants. The microbial genome was found to host five non\u2010ribosomal peptide synthetase (NRPS) gene clusters containing starter condensation domains that direct lipopeptide biosynthesis. Genomics and mass spectrometry (MS)\u2010based metabolomics enabled the linking of two NRPS gene clusters to the corresponding lipopeptide families, leading to the identification of 20 new cyclolipopeptides, designated as rhodoheptins, and 33 new glycolipopeptides, designated as rhodamides. An integrated\n                    <jats:italic>in silico<\/jats:italic>\n                    gene cluster and high\u2010resolution MS\/MS data analysis allowed us to elucidate the planar structure, inference of stereochemistry and reconstruction of the biosynthesis of rhodoheptins and rhodamides. Rhodoheptins are cyclic heptapeptides where the N\u2010terminus is bonded to a \u03b2\u2010hydroxy fatty acid forming a macrolactone ring with the C\u2010terminal amino acid residue. Rhodamides are linear 14\u2010mer glycolipopeptides with a serine\u2010 and alanine\u2010rich peptide backbone, featuring a distinctive pattern of acetylation, glycosylation and succinylation. These molecules exhibited biosurfactant activity in the oil\u2010spreading assay and showed moderate antiproliferative effects against human A375 melanoma cells.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70011","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T02:46:32Z","timestamp":1732502792000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Integrated genome and metabolome mining unveiled structure and biosynthesis of novel lipopeptides from a deep\u2010sea\n                    <i>Rhodococcus<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Costanza","family":"Ragozzino","sequence":"first","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology Stazione Zoologica Anton Dohrn, Giardini del Molosiglio  Naples Italy"},{"name":"Department of Chemical, Biological, Pharmaceutical and Environmental Sciences University of Messina  Messina Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fortunato","family":"Palma\u00a0Esposito","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology Stazione Zoologica Anton Dohrn, Giardini del Molosiglio  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8327-954X","authenticated-orcid":false,"given":"Carmine","family":"Buonocore","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology Stazione Zoologica Anton Dohrn, Giardini del Molosiglio  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Tedesco","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology Stazione Zoologica Anton Dohrn, Giardini del Molosiglio  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Coppola","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology Stazione Zoologica Anton Dohrn, Giardini del Molosiglio  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Paccagnella","sequence":"additional","affiliation":[{"name":"Interfaculty Institute of Microbiology and Infection Medicine Tuebingen, Microbiology\/Biotechnology, University of Tuebingen  Tuebingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Ziemert","sequence":"additional","affiliation":[{"name":"Interfaculty Institute of Microbiology and Infection Medicine Tuebingen, Microbiology\/Biotechnology, University of Tuebingen  Tuebingen Germany"},{"name":"German Centre for Infection Research (DZIF)  T\u00fcbingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8957-3259","authenticated-orcid":false,"given":"Gerardo","family":"Della\u00a0Sala","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology Stazione Zoologica Anton Dohrn, Giardini del Molosiglio  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"de de\u00a0Pascale","sequence":"additional","affiliation":[{"name":"Department of Ecosustainable Marine Biotechnology Stazione Zoologica Anton Dohrn, Giardini del Molosiglio  Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad344"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv171"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15020700"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/md18050269"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.417"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2009.11.027"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2020.124827"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15082156"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010022\u201003323\u20103"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.52.710"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2010.08.001"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28207127"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11274\u2010020\u201002970\u20108"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.00397"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep10316"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42770\u2010023\u201000905\u20107"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074\u20105521(00)00011\u20109"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2022.128287"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00485\u201007"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jasms.9b00059"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioadv\/vbab016"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15082139"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21176138"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/applmicrobiol4010015"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2011.11.062"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1074\u20105521(02)00252\u20101"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2020.183395"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2019.102061"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057991"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0021\u20109673(00)94553\u20109"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2010.06.015"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9102030"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.S120001025"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01545\u201005"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111904"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.3c00591"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010020\u20100933\u20106"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22169055"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00294"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2008.03816.x"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2023.108210"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201011\u2010395"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201402384"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102148\u20107\u201078"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki885"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0026261718060152"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coesh.2020.09.002"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1049"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.2495\/SDP\u2010V11\u2010N5\u2010804\u2010811"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13070471"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.9.2823\u20102830.2003"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20070424"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29112606"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010018\u20100068\u20106"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742\u20104658.2008.06664.x"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589\u2010019\u20100385\u20104"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2np00044j"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201020548\u20108"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T13:58:25Z","timestamp":1762264705000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":59,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1111\/1751-7915.70011"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70011","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"2024-05-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70011"}}