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The electron microscopy analyses showed the production of nanoparticles were intracellular and the resulting particles were extracted and characterized by XRD, zeta potential, FESEM, EDX, FTIR spectroscopy and DLS. These analyses showed amorphous spherical nanoparticles with an average size of 110\u2009nm and a Zeta potential of \u221234.51\u2009\u00b1\u20092.41\u2009mV. Signatures of lipids and proteins were present in the capping layer of biogenic selenium nanoparticles based on FTIR spectra. The antimicrobial properties of test strains showed that\n                    <jats:italic>Serratia marcescens<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumonia<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    were inhibited at concentrations between 160 and 640\u2009\u03bcg\/mL, while the growth of\n                    <jats:italic>Candida albicans was hindered by<\/jats:italic>\n                    80\u2009\u03bcg\/mL of biogenic selenium nanoparticles. At concentrations between 0.5 and 1.5\u2009mg\/mL of biogenic selenium nanoparticles inhibited up to 50% of biofilm formation of\n                    <jats:italic>Klebsiella pneumonia<\/jats:italic>\n                    ,\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    ,\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . Additionally, the concentration of 20\u2013640\u2009\u03bcg\/mL of these bioSeNPs showed antioxidant activity. Evaluating the cytotoxicity of these nanoparticles on the HUVEC and HepG2 cell lines did not show any significant toxicity within MIC concentrations of SeNPs. This defines that\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    synthesized SeNPs have strong potential to be exploited as antimicrobial agents against pathogens of WHO concern.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70013","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T05:05:05Z","timestamp":1728018305000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Characterization and biological activity of selenium nanoparticles biosynthesized by\n                    <i>Yarrowia lipolytica<\/i>"],"prefix":"10.1111","volume":"17","author":[{"given":"Elham","family":"Lashani","sequence":"first","affiliation":[{"name":"Extremophiles Laboratory, Department of Microbiology, School of Biology, College of Science University of Tehran  Tehran 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