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By disrupting the formate dehydrogenase encoding\n                    <jats:italic>FDH1<\/jats:italic>\n                    gene, we converted such a system into a self\u2010induced one, as adding any inducer in the culture medium is no longer requested for p\n                    <jats:italic>AOX1<\/jats:italic>\n                    induction. In cells, formate is generated from serine through the THF\u2010C1 metabolism, and it cannot be converted into carbon dioxide in a FdhKO strain. Under non\u2010repressive culture conditions, such as on sorbitol, the intracellular formate generated from the THF\u2010C1 metabolism is sufficient to induce p\n                    <jats:italic>AOX1<\/jats:italic>\n                    and initiate protein synthesis. This was evidenced for two model proteins, namely intracellular eGFP and secreted CalB lipase from\n                    <jats:italic>C.\u2009antarctica.<\/jats:italic>\n                    Similar protein productivities were obtained for a FdhKO strain on sorbitol and a non\u2010disrupted strain on sorbitol\u2010methanol. Considering a\n                    <jats:italic>K.\u2009Phaffii<\/jats:italic>\n                    FdhKO strain as a workhorse for recombinant protein synthesis paves the way for the further development of methanol\u2010free processes in\n                    <jats:italic>K.\u2009phaffii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70022","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T12:38:35Z","timestamp":1728304715000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Formate from\n                    <scp>THF<\/scp>\n                    \u2010\n                    <scp>C1<\/scp>\n                    metabolism induces the\n                    <i>AOX1<\/i>\n                    promoter in formate dehydrogenase\u2010deficient\n                    <i>Komagataella phaffii<\/i>"],"prefix":"10.1111","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9503-2993","authenticated-orcid":false,"given":"Cristina","family":"Bustos","sequence":"first","affiliation":[{"name":"Microbial Processes and Interactions, TERRA Teaching and Research Centre, Gembloux Agro\u2010Bio Tech University of Liege  Gembloux Belgium"},{"name":"School of Biochemical Engineering Pontificia Universidad Cat\u00f3lica de Valpara\u00edso  Valparaiso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6180-0023","authenticated-orcid":false,"given":"Julio","family":"Berrios","sequence":"additional","affiliation":[{"name":"School of Biochemical Engineering Pontificia Universidad Cat\u00f3lica de Valpara\u00edso  Valparaiso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2600-5833","authenticated-orcid":false,"given":"Patrick","family":"Fickers","sequence":"additional","affiliation":[{"name":"Microbial Processes and Interactions, TERRA Teaching and Research Centre, Gembloux Agro\u2010Bio Tech University of Liege  Gembloux 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