{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:45:10Z","timestamp":1790639110306,"version":"4.1.0"},"reference-count":72,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T00:00:00Z","timestamp":1732147200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["RS\u20102023\u201000304637"],"award-info":[{"award-number":["RS\u20102023\u201000304637"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/Y0042X\/1"],"award-info":[{"award-number":["EP\/Y0042X\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas putida<\/jats:italic>\n                    <\/jats:styled-content>\n                    is widely used in industrial applications, including the recombinant proteins production, because of its natural advantageous properties. In this study, the gene encoding FleQ, the primary regulator of flagellar synthesis, was deleted to construct a new non\u2010motile\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. putida<\/jats:italic>\n                    <\/jats:styled-content>\n                    KT2440\u2010derived strain (\n                    <jats:italic>\u0394fleQ<\/jats:italic>\n                    ). The non\u2010motile cells showed reduced biofilm formation and enhanced expression of a heterologous gene in nutrient\u2010rich media compared with the wild\u2010type (WT) strain, attributed to the reallocation of cellular resources from flagellar synthesis and cellular motility. Additionally, the\n                    <jats:italic>\u0394fleQ<\/jats:italic>\n                    strain exhibited enhanced tolerance to chloramphenicol, indicating higher ribosome production, confirmed by a higher RNA\/protein ratio relative to the WT. While the WT strain showed decreased growth and a three\u2010fold increase in reporter gene activity in minimal media, the\n                    <jats:italic>\u0394fleQ<\/jats:italic>\n                    strain maintained consistent reporter gene expression and exhibited a relatively higher growth rate. This suggests that the FleQ is involved in modulating proteome allocation based on nutrient quality. The removal of FleQ allows for more flexible resource allocation, creating a chassis strain with nutrient quality\u2010independent gene expression capacity, which could be valuable in industrial applications where consistent output is essential.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70054","type":"journal-article","created":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T13:39:44Z","timestamp":1732196384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Impact of\n                    <scp>\n                      <i>fleQ<\/i>\n                    <\/scp>\n                    Deficiency on Resource Allocation and Heterologous Gene Expression in\n                    <scp>\n                      <i>Pseudomonas putida<\/i>\n                    <\/scp>\n                    Across Various Growth Media"],"prefix":"10.1111","volume":"17","author":[{"given":"Junyoung","family":"Kim","sequence":"first","affiliation":[{"name":"School of Life Sciences BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sooyeon","family":"Lee","sequence":"additional","affiliation":[{"name":"School of Life Sciences BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander P. S.","family":"Darlington","sequence":"additional","affiliation":[{"name":"Warwick Integrative Synthetic Biology Centre, School of Engineering University of Warwick  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2620-8104","authenticated-orcid":false,"given":"Juhyun","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Life Sciences BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07415.x"},{"key":"e_1_2_9_3_1","first-page":"e01053","article-title":"Microbial diversification is maintained in an experimentally evolved synthetic community","author":"Al\u2010Tameemi 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