{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T04:17:22Z","timestamp":1779164242892,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T00:00:00Z","timestamp":1747958400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1800200"],"award-info":[{"award-number":["2022YFD1800200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    (\n                    <jats:italic>T. gondii<\/jats:italic>\n                    ), a parasitic protozoan capable of infecting nearly all warm\u2010blooded animals, causes significant economic losses in livestock and poses a significant threat to both animal and public health. Despite its impact, no ideal vaccine is currently available to prevent toxoplasmosis. Vesicular transport plays a crucial role in the life cycle of\n                    <jats:italic>T.\u2009gondii<\/jats:italic>\n                    , and proteins involved in this process \u2013 such as those containing C2 domains \u2013 may serve as novel targets for the development of live attenuated vaccines. In this study, we evaluated the feasibility of a C2 domain\u2010containing protein (TGME49_203240) as a live attenuated vaccine candidate. Our findings suggest that TGME49_203240 may be involved in vesicular transport and that it is essential for\n                    <jats:italic>T.\u2009gondii<\/jats:italic>\n                    growth. Deletion of TGME49_203240 reduced parasite virulence and impaired tissue cyst formation in mice. Moreover, mice vaccinated with ME49\n                    <jats:italic>\u0394203240<\/jats:italic>\n                    were protected against the lethal challenge of the tachyzoites of\n                    <jats:italic>T.\u2009gondii<\/jats:italic>\n                    I, II, III strains and cysts of II strain. In addition, the ME49\n                    <jats:italic>\u0394203240<\/jats:italic>\n                    strain elicited robust immune responses, including the production of high levels of specific IgG antibodies and key cytokines (IFN\u2010\u03b3, TNF\u2010\u03b1 and IL\u201012). These findings highlight TGME49_203240 as a promising target for the development of a live attenuated vaccine against\n                    <jats:italic>T.\u2009gondii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70143","type":"journal-article","created":{"date-parts":[[2025,5,23]],"date-time":"2025-05-23T09:40:16Z","timestamp":1747993216000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>Toxoplasma gondii<\/i>\n                    <scp>C2<\/scp>\n                    Domain Protein Deletion Mutant as a Promising Vaccine Against Toxoplasmosis in Mice"],"prefix":"10.1111","volume":"18","author":[{"given":"Yifan","family":"Luo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine Huazhong Agricultural University  Wuhan Hubei 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