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The enzymes involved in the oxidation to FDCA, PsfA and PsfG, were identified and characterised. Deletion of FDCA decarboxylase HmfF involved in the conversion of FDCA to furoic acid, and subsequently to a central metabolic intermediate, 2\u2010ketoglutarate, allowed for the accumulation of FDCA. GO16 \u0394\n                    <jats:italic>hmfF<\/jats:italic>\n                    cells were grown on glycerol, TPA, EG or mock PET hydrolysate, and the catalyst was then used for the biotransformation of HMF to FDCA. When TPA was used as a growth substrate and to power the biotransformation, the transport of 5\u2010hydroxymethyl\u20102\u2010furancarboxylic acid (HMFCA) into the cytoplasm represented a rate\u2010limiting step in HMF oxidation. De\u2010bottlenecking transport limitations through\n                    <jats:italic>in trans<\/jats:italic>\n                    overexpression of the HMFCA transporter (HmfT) along with the PsfA aldehyde dehydrogenase and PsfG alcohol dehydrogenase allowed 100% conversion of 50\u2009mM HMF to FDCA within 24\u2009h when TPA, EG or mock PET hydrolysate were used to grow the biocatalyst and subsequently to power the biotransformation. This expands the repertoire of valuable products obtained from engineered\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. umsongensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    GO16 in the strategy to bio\u2010upcycle post\u2010consumer PET.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70159","type":"journal-article","created":{"date-parts":[[2025,5,10]],"date-time":"2025-05-10T04:05:29Z","timestamp":1746849929000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterisation and Harnessing of 5\u2010Hydroxymethylfurfural Metabolism in\n                    <scp>\n                      <i>Pseudomonas umsongensis<\/i>\n                      GO16\n                    <\/scp>\n                    for the Production of 2,5\u2010Furandicarboxylic Acid"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0290-6431","authenticated-orcid":false,"given":"Rhys","family":"Orimaco","sequence":"first","affiliation":[{"name":"UCD Earth Institute and School of Biomolecular and Biomedical Science University College Dublin  Dublin 4 Ireland"},{"name":"BiOrbic \u2010 Bioeconomy Research Centre Ireland, University College Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauric","family":"Donnelly","sequence":"additional","affiliation":[{"name":"UCD Earth Institute and School of Biomolecular and Biomedical Science University College Dublin  Dublin 4 Ireland"},{"name":"BiOrbic \u2010 Bioeconomy Research Centre Ireland, University College Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u00e1n","family":"Sexton","sequence":"additional","affiliation":[{"name":"UCD Earth Institute and School of Biomolecular and Biomedical Science 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