{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T19:19:57Z","timestamp":1792869597393,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T00:00:00Z","timestamp":1750032000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014996","name":"Health Commission of Zhejiang Province","doi-asserted-by":"publisher","award":["2019ZD023"],"award-info":[{"award-number":["2019ZD023"]}],"id":[{"id":"10.13039\/501100014996","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Use matrix\u2010assisted laser desorption ionisation time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS) to screen the specific mass peaks of carbapenem\u2010resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    (CRKP), compare the differences in spectrum peaks between intestinal and bloodstream screening of CRKP, and assess the utility of MALDI\u2010TOF MS in quickly identifying various CRKP sources. From 2014 to 2023, a total of 267\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    strains were collected at Quzhou People's Hospital, including 60 intestinal screening isolates from ICU patients and 207 bloodstream infection isolates. MALDI\u2010TOF MS was used to profile peptides in CRKP and carbapenem\u2010sensitive\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    (CSKP), followed by analysis with flexAnalysis and ClinProTools 3.0. Statistically significant protein peaks were selected to build classification models, which were verified using non\u2010duplicate strains. MALDI\u2010TOF MS achieved &gt;\u200999.9% accuracy in identifying\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Characteristic peaks (2523.43, 3041.62, 4520.11, 10,079.18 Da) were used to develop resistance analysis models, with the optimal model (SNN) showing 90.08% sensitivity, 95.80% specificity and identification accuracies of 90% for CSKP and 89.66% for CRKP. Another model using peaks (8876, 8993, 9139 Da) differentiated CRKP origins, with the ideal model (QC) achieving 86.85% sensitivity, 88.46% specificity, and accuracies of 81.82% for bloodstream and 95.00% for intestinal CRKP.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70184","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T09:44:04Z","timestamp":1750067044000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Rapid Identification and Typing of Carbapenem\u2010Resistant\n                    <i>Klebsiella pneumoniae<\/i>\n                    Using\n                    <scp>MALDI<\/scp>\n                    \u2010\n                    <scp>TOF MS<\/scp>\n                    and Machine 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