{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T01:14:30Z","timestamp":1781918070239,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32000088"],"award-info":[{"award-number":["32000088"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32060024"],"award-info":[{"award-number":["32060024"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Carbapenem resistance driven by metallo\u2010\u03b2\u2010lactamases (MBLs) poses a formidable global challenge as these enzymes can degrade a wide range of \u03b2\u2010lactam antibiotics, including last\u2010line carbapenems. Despite extensive documentation of MBL\u2010producing pathogens, their evolutionary origins and ecological reservoirs are still poorly understood. Here, we report the discovery and in\u2010depth characterisation of AMM\u20101, a previously unrecognised B1.2 MBL identified within a metagenome\u2010assembled genome of\n                    <jats:italic>Alteromonas mangrovi<\/jats:italic>\n                    obtained from the Yangtze River Estuary. Comparative sequence analyses and phylogenetics reveal that AMM\u20101 clusters closely with clinically significant MBLs, underscoring its potential impact to human health. Structural modelling confirms the presence of a conserved di\u2010zinc binding site critical for \u03b2\u2010lactam hydrolysis, while heterologous expression in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) demonstrates a marked increase in resistance against multiple \u03b2\u2010lactam classes, including carbapenems. Phylogenetic depth analysis and ancestral reconstruction delineate AMM\u20101's distinct evolutionary path, placing it deeper than IMP\u20101 and SPM\u20101 but shallower than NDM\u20101. Flexibility simulations reveal unique active\u2010site loop dynamics (L3 and L10), with reduced mobility in key regions that shape substrate binding stability and spectrum. Notably, AMM\u20101 is stably located on the host chromosome without flanking mobile genetic elements, suggesting that it may have persisted as a vertically inherited trait rather than a recently acquired component of a mobile resistome. These findings highlight the capacity of environmental microbes to serve as long\u2010standing, cryptic reservoirs of potent resistance determinants, emphasising the need for integrated environmental surveillance and preemptive stewardship strategies. By unveiling the molecular and functional properties of AMM\u20101, this work provides critical insights into how resistance elements can reside, evolve and potentially mobilise within natural habitats, ultimately informing efforts to predict and mitigate the future emergence of carbapenem\u2010resistant bacterial pathogens.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70191","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T14:25:30Z","timestamp":1752071130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Novel Metallo\u2010\u03b2\u2010Lactamase\n                    <scp>AMM<\/scp>\n                    \u20101 From\n                    <scp>\n                      <i>Alteromonas mangrovi<\/i>\n                    <\/scp>\n                    Reveals a Cryptic Environmental Reservoir of Carbapenem Resistance"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9048-4722","authenticated-orcid":false,"given":"Xuan","family":"Wu","sequence":"first","affiliation":[{"name":"Key Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing Chongqing Ecological and Environment Monitoring Center  Chongqing China"},{"name":"College of Environment and Ecology (CEE) Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinjing","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Second Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing Chongqing Ecological and Environment Monitoring Center  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ningning","family":"Pi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Environmental Chemistry and Ecotoxicology of Organic Pollutants of Chongqing Chongqing Ecological and Environment Monitoring Center  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine Chongqing Medical University  Chongqing China"},{"name":"Western (Chongqing) Institute for Digital\u2010Intelligent Medicine, Chongqing National Biomedicine Industry Park  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Xiang","sequence":"additional","affiliation":[{"name":"Western (Chongqing) Institute for Digital\u2010Intelligent Medicine, Chongqing National Biomedicine Industry Park  Chongqing China"},{"name":"Precision Medicine Center The Second Affiliated Hospital of Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.01275-22"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3439"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa392"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-019-0670-1"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix893"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-020-00832-7"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.14510"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz848"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-019-0714-6"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.174143"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-020-01545-5"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit355"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29096-9"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2020.0462"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.08.162"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08853-3"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2019.05.074"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.1035"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-19279-0"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1023948"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-017-1012-8"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.697"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku433"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00649-x"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2016.02.020"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2021.117447"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e28796"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2017.07.060"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2019.113143"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01090\u201001021"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29163944"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aau9124"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02032-15"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26185489"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki065"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/w12123313"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2014.04.034"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3101.240722"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.04.002"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.306-325.2005"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-024-03365-7"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00115-18"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00261-22"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvac080"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2012.12.008"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks261"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18475-9"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiaa130"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.146009"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.201900221"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.270"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48219-y"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70191","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:36:10Z","timestamp":1762252570000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":56,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/1751-7915.70191"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70191","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-06-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70191"}}