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In particular, biotechnological processes which use one carbon (C1) compounds as feedstock represent an interesting avenue. Many bacterial species evolved naturally to thrive on such compounds, among them\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Cupriavidus necator<\/jats:styled-content>\n                      ,\n                    <\/jats:italic>\n                    which has been studied in the past due to its range of metabolic capabilities in utilisation and production of compounds of interest.\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cupriavidus necator<\/jats:italic>\n                    <\/jats:styled-content>\n                    strain H16 is the reference laboratory strain for this species and by far the most extensively studied. In contrast, research efforts and genomic characterisation of other strains within this species have been limited and sporadic. Therefore, the genomic diversity and full metabolic potential across the broader species remain poorly understood. In this work, we collected publicly available genomes along with newly sequenced ones. From a collection of 44 genomes, we curated a final collection of 22 genomes deemed to be\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. necator<\/jats:italic>\n                    <\/jats:styled-content>\n                    . We examined hallmark metabolic functions, including carbon dioxide fixation, formate assimilation and hydrogen utilisation. We identified methylation motifs and restriction modification systems. Finally, strains ATCC 25207, TA06, and 1978 are proposed as candidate strains of interest based on their genomic make\u2010up and observations from literature. This work provides a comprehensive genomic resource for the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. necator<\/jats:italic>\n                    <\/jats:styled-content>\n                    species, facilitating its development as a biomanufacturing platform and advancing our understanding of its metabolic diversity and potential applications.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70201","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T14:45:01Z","timestamp":1753454701000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative Genomic Assessment of the\n                    <scp>\n                      <i>Cupriavidus necator<\/i>\n                    <\/scp>\n                    Species for One\u2010Carbon Based Biomanufacturing"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9751-9877","authenticated-orcid":false,"given":"Magnus G.","family":"Jespersen","sequence":"first","affiliation":[{"name":"The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark  Kongens Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emil Funk","family":"Vangsgaard","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark  Kongens Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Arango","family":"Saavedra","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark  Kongens Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9946-0346","authenticated-orcid":false,"given":"Stefano","family":"Donati","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark  Kongens Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8191-3511","authenticated-orcid":false,"given":"Lars K.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark  Kongens Lyngby Denmark"},{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN) The University of Queensland  Brisbane Queensland Australia"},{"name":"ARcCentre of Excellence in SyntheticBiology The University of Queensland  Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101955"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.076448.108"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.4c00757"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fsufs.2021.681596"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nargab\/lqad066"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2024.10.014"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab203"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00403610"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.53.5.1094-1102.1987"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00388"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.15.4522-4529.1996"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001421"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.001244"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2022.10.016"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002809"},{"key":"e_1_2_9_17_1","doi-asserted-by":"crossref","unstructured":"Crits\u2010Christoph A. 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