{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:55:43Z","timestamp":1790351743239,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T00:00:00Z","timestamp":1754870400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The overwhelming amount of plastic produced is an unprecedented challenge for humanity due to the lack of end\u2010of\u2010life solutions for heterogeneous plastic wastes. One possibility is feedstock recycling of mixed plastics and complex polymers with subsequent biological funnelling and upcycling. Major depolymerisation products of common plastics such as polyurethanes, polyesters and polyamides include aliphatic dicarboxylic acids or diols such as adipic acid (AA) and 1,4\u2010butanediol (BDO), which can be metabolised by engineered\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas putida<\/jats:italic>\n                    <\/jats:styled-content>\n                    strains. However, the spectrum of upcycled compounds that can be produced from these monomers is still limited. Therefore, we extended the substrate spectrum of an aromatics\u2010overproducing\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas taiwanensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    strain to AA and BDO. Adaptive laboratory evolution (ALE) followed by genome sequencing was used to identify and reverse engineer key growth\u2010enabling mutations. In this context, we observed a conflict between the dual objectives of fast growth on AA and efficient aromatics production, which materialised in the form of mutations in the ribosomal protein\u2010encoding gene\n                    <jats:italic>rpmE.<\/jats:italic>\n                    These mutations promote faster growth on AA at the cost of aromatics production. In contrast to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. putida<\/jats:italic>\n                    <\/jats:styled-content>\n                    KT2440, knockout of the repressor gene\n                    <jats:italic>psrA<\/jats:italic>\n                    regulating expression of genes involved in\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010oxidation had no positive effect on growth of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. taiwanensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    on AA. Evolution for growth on BDO revealed several point mutations that affect expression of multiple oxidoreductases, with an identified key role for the dehydrogenase encoded by PVLB_10545. This dehydrogenase likely catalyses the initial oxidation of BDO, thus substituting for PedE, which is present in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. putida<\/jats:italic>\n                    <\/jats:styled-content>\n                    but absent in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. taiwanensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Integration of\n                    <jats:italic>RpcTAL<\/jats:italic>\n                    into the Tn7 site enabled\n                    <jats:italic>de novo<\/jats:italic>\n                    production of 4\u2010coumarate with a yield of 14.4%\u2009\u00b1\u20090.1% (Cmol\/Cmol) from BDO and 11.5%\u2009\u00b1\u20090.3% (Cmol\/Cmol) from AA. Thereby, the potential of these\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. taiwanensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    strains for upcycling plastic hydrolysates to value\u2010added compounds was successfully demonstrated.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70205","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T15:35:21Z","timestamp":1754926521000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Engineering of 1,4\u2010Butanediol and Adipic Acid Metabolism in\n                    <i>Pseudomonas taiwanensis<\/i>\n                    for Upcycling to Aromatic Compounds"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4318-8686","authenticated-orcid":false,"given":"Leonie","family":"Op de Hipt","sequence":"first","affiliation":[{"name":"Institute of Bio\u2010 and Geosciences IBG\u20101: Biotechnology Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannic S.","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Institute of Bio\u2010 and Geosciences IBG\u20101: Biotechnology Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"de Jong","sequence":"additional","affiliation":[{"name":"Institute of Bio\u2010 and Geosciences IBG\u20101: Biotechnology Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tino","family":"Polen","sequence":"additional","affiliation":[{"name":"Institute of Bio\u2010 and Geosciences IBG\u20101: Biotechnology Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedikt","family":"Wynands","sequence":"additional","affiliation":[{"name":"Institute of Bio\u2010 and Geosciences IBG\u20101: Biotechnology Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"Wierckx","sequence":"additional","affiliation":[{"name":"Institute of Bio\u2010 and Geosciences IBG\u20101: Biotechnology Forschungszentrum J\u00fclich  J\u00fclich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010024\u201002310\u20107"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2021.05.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25052957"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20200173"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aba3656"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202200062"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41893\u2010021\u201000807\u20102"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2144\/000112149"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41929\u2010021\u201000648\u20104"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pen.24770"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2018.06.003"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1700782"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/NMETH.1318"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.11.2850\u20102855.1989"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2011.11.022"},{"key":"e_1_2_9_17_1","unstructured":"International Energy Agency (IEA).2022.\u201cWorld Energy Outlook 2022.\u201dhttps:\/\/www.iea.org\/reports\/world\u2010energy\u2010outlook\u20102022."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/d3gc04728h"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260352"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8ee00460a"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201004350\u20100"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aiepr.2020.01.001"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00551"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586\u2010023\u201002175\u20107"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2023.12.010"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2019.00130"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462\u20102920.14703"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00382"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1410657111"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010015\u20106745\u20104"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462\u20102920.2011.02538.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2gc02244c"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.032847\u20100"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/POLYM12040920"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751\u20107915.13712"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2019.00312"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.10.4817\u20104827.2001"},{"key":"e_1_2_9_38_1","unstructured":"PlasticsEurope.2022.\u201cPlastics \u2013 The Facts 2022: An Analysis of European Plastics Production Demand and Waste Data.\u201d"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04988\u201022"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201900569"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201005422\u20105"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abo4626"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.0c05651"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2020.00976"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2023.10.005"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2010.08.001"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00570\u201017"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751\u20107915.12312"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010environ\u2010102016\u2010060700"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/catal13020340"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2023.05.004"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2018.03.011"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.9b00108"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssynbio.5b00058"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:54:14Z","timestamp":1762275254000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":53,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/1751-7915.70205"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70205","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"2025-02-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70205"}}