{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:18:50Z","timestamp":1780359530156,"version":"3.54.1"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Clostridium perfringens<\/jats:italic>\n                    <\/jats:styled-content>\n                    is a multi\u2010host opportunistic pathogen whose plasmid\u2010encoded toxins cause gas gangrene, necrotic enteritis and enterotoxemia, resulting in substantial economic losses in animal husbandry. In light of antibiotic bans and the need for alternatives, we employed reverse network pharmacology to screen and in\u00a0vitro validate artemisinin (ART), then assessed its efficacy in murine and rabbit infection models challenged with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. perfringens<\/jats:italic>\n                    <\/jats:styled-content>\n                    type F. ART treatment did not significantly affect body weight change or intestinal histopathological damage. However, it significantly modulated inflammatory cytokines and antioxidant parameters in a tissue\u2010 and species\u2010dependent manner. Specifically, ART increased serum TNF\u2010\u03b1 in mice, decreased IL\u20101\u03b2 in rabbits and elevated IL\u201010 in multiple tissues. In addition, ART enhanced hepatic SOD and T\u2010AOC in mice and reduced hepatic MDA in rabbits. Microbiota analysis revealed limited and subtle shifts in community structure following ART intervention. Transcriptomic analysis further indicated that ART treatment induced marked changes in hepatic gene expression, particularly involving detoxification, lipid metabolism and stress response pathways, with notable species\u2010specific differences in enrichment profiles. While correlation analysis suggested associations of\n                    <jats:italic>Anaerotruncus<\/jats:italic>\n                    with hepatic detoxification genes and\n                    <jats:italic>Bacteroides<\/jats:italic>\n                    with inflammation\u2010regulatory genes, these genus\u2010level findings are based on correlation only and should be interpreted with caution given the lack of significant changes in overall microbial community structure. Collectively, these results indicate that ART can modulate host inflammatory and antioxidant responses, but its impact on gut microbiota composition in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. perfringens<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection models appears limited, and the biological significance of observed genus\u2010level associations remains to be elucidated.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70235","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T14:56:58Z","timestamp":1761749818000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Liver\u2013Microbiome Crosstalk Mediates the Protective Effects of Artemisinin in\n                    <i>Clostridium perfringens<\/i>\n                    Models"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-9568-132X","authenticated-orcid":false,"given":"Haodong","family":"Han","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoya","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi 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