{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T01:35:05Z","timestamp":1781919305807,"version":"3.54.5"},"reference-count":52,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:00:00Z","timestamp":1760140800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ethanol stress poses a considerable challenge for\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    during fermentation. Strains carrying an extra copy of chromosome III exhibit enhanced ethanol tolerance. Here, we investigated the underlying mechanisms of this tolerance, focusing on gene dosage effects and differential gene expression under ethanol stress. We compared the gene expression profiles of a strain with three copies of chromosome III and its derivative with two copies, exposed to 6% and 10% ethanol. Our analysis identified\n                    <jats:italic>TUP1<\/jats:italic>\n                    , located on chromosome III, as a key regulator of the ethanol stress response. Deleting one copy of\n                    <jats:italic>TUP1<\/jats:italic>\n                    in the tolerant strain diminished its ethanol tolerance, suggesting that chromosome III aneuploidy in ethanol\u2010tolerant strains enhances adaptive responses by increasing\n                    <jats:italic>TUP1<\/jats:italic>\n                    copy number. Our findings offer insights into the genetic basis of ethanol tolerance, with potential applications for optimising industrial fermentation processes and understanding the role of aneuploidy in the domestication of industrial yeasts.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70244","type":"journal-article","created":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:30:24Z","timestamp":1760167824000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chromosome\n                    <scp>III<\/scp>\n                    Aneuploidy Enhances Ethanol Tolerance in Industrial\n                    <i>Saccharomyces cerevisiae<\/i>\n                    by Increasing the\n                    <scp>\n                      <i>TUP1<\/i>\n                    <\/scp>\n                    Copy Number"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9459-1870","authenticated-orcid":false,"given":"Sonia","family":"Albillos\u2010Arenal","sequence":"first","affiliation":[{"name":"Yeastomics Laboratory (SBYBI Group) Food Biotechnology Department, IATA\u2010 CSIC  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6474-9040","authenticated-orcid":false,"given":"Javier","family":"Alonso del Real","sequence":"additional","affiliation":[{"name":"Yeastomics Laboratory (SBYBI Group) Food Biotechnology Department, IATA\u2010 CSIC  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cristina","family":"Adam","sequence":"additional","affiliation":[{"name":"Yeastomics Laboratory (SBYBI Group) Food Biotechnology Department, IATA\u2010 CSIC  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1024-954X","authenticated-orcid":false,"given":"Eladio","family":"Barrio","sequence":"additional","affiliation":[{"name":"Yeastomics Laboratory (SBYBI Group) Food Biotechnology Department, IATA\u2010 CSIC  Valencia Spain"},{"name":"Department of Genetics University of Valencia (UV)  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6478-6845","authenticated-orcid":false,"given":"Amparo","family":"Querol","sequence":"additional","affiliation":[{"name":"Yeastomics Laboratory (SBYBI Group) Food Biotechnology Department, IATA\u2010 CSIC  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185516"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02503-0"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2017.03.009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010559"},{"key":"e_1_2_9_7_1","first-page":"27","article-title":"Yeast Stress Response and Fermentation Efficiency: How to Survive the Making of Wine\u2014A Review","volume":"21","author":"Bauer E.","year":"2000","journal-title":"South African Journal of Enology and Viticulture"},{"key":"e_1_2_9_8_1","first-page":"85","volume-title":"Biodiversity and Biotechnology of Wine Yeasts","author":"Bisson L. F.","year":"2002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E20-11-0715"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12155\u2010014\u20109533\u20106"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2008.02.002"},{"key":"e_1_2_9_12_1","first-page":"427","article-title":"A Genomic Approach to Defining the Ethanol Stress Response in the Yeast \n                     Saccharomyces cerevisiae","volume":"54","author":"Chandler M.","year":"2004","journal-title":"Annales de Microbiologie"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.141952.112"},{"key":"e_1_2_9_14_1","article-title":"RegulatorDB: A Resource for the Analysis of Yeast Transcriptional Regulation","volume":"2017","author":"Choi J. A.","year":"2017","journal-title":"Database: The Journal of Biological Databases and Curation"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.22.15350"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-009-2223-1"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.08.020"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz931"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1385\/1\u201059259\u2010958\u20103:107"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.3427"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2593"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.106.065292"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.16157"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00440-21"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2000.01017.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1010876"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113672"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4456-4461.2000"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010010\u20102594\u20103"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19990915)15:12<1211::AID-YEA448>3.0.CO;2-H"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.6.1288\u20101303.2003"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00082"},{"key":"e_1_2_9_35_1","first-page":"135","article-title":"Natural Proteome Diversity Links Aneuploidy Tolerance to Protein Turnover","volume":"625","author":"Muenzner J.","year":"2024","journal-title":"Nature"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008115"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005809"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyab120"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(02)00557-9"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.58.9.2948-2953.1992"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02814733"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0259476"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04657.x"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.11.001"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1041"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4218"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1553"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01263-15"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005635"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.179275.111"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2012.00818.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1211150109"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.6.1875-1881.1990"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70244","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T12:09:19Z","timestamp":1761653359000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70244"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":52,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/1751-7915.70244"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70244","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70244"}}