{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T11:00:10Z","timestamp":1784631610496,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["KY13022407"],"award-info":[{"award-number":["KY13022407"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20241953"],"award-info":[{"award-number":["BK20241953"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701390"],"award-info":[{"award-number":["81701390"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The gut\u2010testis axis enables gut microbes to influence host reproduction; nonetheless, the specific role of microbial genetic variation in this process remains elusive. In this study, using\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) as a model organism, we identified 46\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) strains that markedly enhanced\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    fertility. Of them, 26 strains were mutant variants capable of mitigating cyclophosphamide (CTX)\u2010induced reproductive disorders in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    . To investigate their application, we constructed probiotics to validate their effectiveness in mouse reproduction. The engineering probiotic Ecn \u0394\n                    <jats:italic>pal<\/jats:italic>\n                    significantly improved spermatogenesis in mice with CTX\u2010induced reproductive disorders. Finally, comprehensive metabolome and transcriptome analysis suggested that the purine metabolism pathway may contribute to ameliorating cyclophosphamide\u2010induced male reproductive toxicity. Overall, our study provides novel insights into the impact of gut microbial genetic variation on host reproduction and elucidates novel therapeutic avenues for mitigating\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6350852\/\">CTX\u2010induced<\/jats:ext-link>\n                    male reproductive toxicity.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70248","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T09:57:14Z","timestamp":1760090234000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gut Microbial Genetic Variation Regulates Host Reproduction"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6112-7230","authenticated-orcid":false,"given":"Xiaoyue","family":"Ding","sequence":"first","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalun","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianshuang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongrong","family":"Gu","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Cai","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Ren","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"Tan","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Medical Technology College Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuobin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Engineering Research Center for Precision Diagnosis and Treatment of Polygenic Critical Diseases, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.4103\/0971\u20106580.97224"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2016.68"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/msb4100050"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/etc.5620220518"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007545"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.977574"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.2.496"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20010031"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19981115)83:10<2217::AID-CNCR22>3.0.CO;2-J"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.06.013"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.04.026"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00028\u201022"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201025315\u2010x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201000974\u20104"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep224"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2016.05.004"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2017.08.019"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612805666230110215458"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.049"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2000108117"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2015.11.005"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.07486\u201011"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.867011"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0223892"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113122"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.108.006437"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084877"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2016.03.006"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnw212"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20100878\u2010z"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.13143"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2025.03.039"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2017.01.008"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.07.010"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70248","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T12:09:31Z","timestamp":1761653371000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":34,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/1751-7915.70248"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70248","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"2025-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70248"}}