{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T16:04:29Z","timestamp":1782835469101,"version":"3.54.5"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2025ZFJH03"],"award-info":[{"award-number":["2025ZFJH03"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["QN25H030026"],"award-info":[{"award-number":["QN25H030026"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pediococcus pentosaceus<\/jats:italic>\n                    <\/jats:styled-content>\n                    Li05, a strain of lactic acid bacteria isolated from the faeces of healthy volunteers, exhibited potential protective effects against various diseases. This study performed third\u2010generation sequencing and detailed characterisation of its genome. The Li05 chromosome harboured conserved genes associated with acid resistance (\n                    <jats:italic>atp<\/jats:italic>\n                    ), bile salt resistance (\n                    <jats:italic>bsh<\/jats:italic>\n                    ), oxidative stress resistance (\n                    <jats:italic>hsl<\/jats:italic>\n                    ,\n                    <jats:italic>dltA<\/jats:italic>\n                    , and et\u00a0al.), and adhesion (\n                    <jats:italic>nrd<\/jats:italic>\n                    ,\n                    <jats:italic>gap<\/jats:italic>\n                    , and et\u00a0al.), whereas the plasmid did not contain antibiotic resistance or virulence genes. Following intervention with Li05 in loperamide\u2010induced constipated mice, constipation symptoms improved. Meanwhile, alterations in gut microbiota, increased BSH activity in faeces, and modifications to the faecal bile acid profile were observed. Additionally, expression levels of TGR5 and TPH1 in the colon of the mice increased, leading to elevated 5\u2010HT levels. When the\n                    <jats:italic>TGR5<\/jats:italic>\n                    gene was knocked out or the TPH1 inhibitor LX1606 was administered to suppress 5\u2010HT synthesis in constipated mice, the beneficial effects of Li05 on gastrointestinal motility and mucus secretion were reversed. Culturing intestinal organoids demonstrated that increased bile acids such as DCA, Iso\u2010LCA, and EALCA could enhance 5\u2010HT levels through the TGR5\/TPH1 axis. Therefore, we concluded that Li05 regulated bile acid metabolism, subsequently increasing 5\u2010HT levels through the TGR5\/TPH1 axis, thus alleviating constipation.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70257","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:40:42Z","timestamp":1761763242000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Protective Effect of\n                    <i>Pediococcus pentosaceus<\/i>\n                    Li05 on Constipation via TGR5\/TPH1\/5\u2010HT Activation"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3180-5595","authenticated-orcid":false,"given":"Hui","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious 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