{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:45:36Z","timestamp":1788273936332,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100024250","name":"Israel Innovation Authority","doi-asserted-by":"publisher","award":["64948"],"award-info":[{"award-number":["64948"]}],"id":[{"id":"10.13039\/501100024250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Monitoring acetic acid (AC) in fermentation processes is essential as excessive AC accumulation, particularly during alcoholic fermentation, can disrupt fermentation and lead to spoilage. However, conventional detection methods such as steam distillation, GC\u2013MS, and HPLC are costly, time\u2010consuming, and require liquid\u2010phase samples, limiting their use for real\u2010time monitoring and early identification of AC buildup. Here, we present an alternative tool for AC detection using a whole\u2010cell bacterial biosensor, which utilises the YwbIR transcriptional regulator from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacillus subtilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . The designed biosensor exhibits high sensitivity, manifesting a linear response with (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009=\u20090.97) from 0 to 1.0\u2009g\/L and a 5\u20138 fold induction at wine spoilage\u2010relevant concentrations. It retains functionality in ethanol\u2010rich matrices (up to 14.5% v\/v) and enables headspace detection. Specificity assays and molecular docking analyses confirm high affinity for AC over other volatile fatty acids. This biosensor offers a low\u2010cost solution for real\u2010time AC monitoring, allowing timely intervention before spoilage occurs and supporting improved quality assurance in fermentation\u2010driven food and beverage production.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70267","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T17:42:09Z","timestamp":1766166129000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of Spoilage\u2010Associated Acetic Acid Levels Using a Transcription\u2010Based Whole\u2010Cell Biosensor"],"prefix":"10.1111","volume":"18","author":[{"given":"Yulia Melnik","family":"Kesler","sequence":"first","affiliation":[{"name":"The Institute of Environmental Sciences (IES), Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor","family":"Kviatkovski","sequence":"additional","affiliation":[{"name":"The Institute of Environmental Sciences (IES), Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neta","family":"Rotem","sequence":"additional","affiliation":[{"name":"The Institute of Environmental Sciences (IES), Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Brandis","sequence":"additional","affiliation":[{"name":"Tevie Mehlman, Life Sciences Core Facilities Weizmann Institute of Science  Rehovot Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oded","family":"Shoseyov","sequence":"additional","affiliation":[{"name":"The Institute of Plant Sciences, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tali","family":"Yarnitzky","sequence":"additional","affiliation":[{"name":"Scientific Consulting  Modi'in\u2010Macabim\u2010Reut Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0383-2809","authenticated-orcid":false,"given":"Yael","family":"Helman","sequence":"additional","affiliation":[{"name":"The Institute of Environmental Sciences (IES), Department of Plant Pathology and Microbiology, The Robert H. 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