{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T15:24:53Z","timestamp":1764170693960,"version":"3.46.0"},"reference-count":56,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017702","name":"Innovation and Entrepreneurship Talent Project of Lanzhou","doi-asserted-by":"publisher","award":["2023\u2010RC\u20103"],"award-info":[{"award-number":["2023\u2010RC\u20103"]}],"id":[{"id":"10.13039\/501100017702","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Despite the availability of vaccines, foot\u2010and\u2010mouth disease (FMD) remains a significant concern in many developing countries, causing severe economic losses and affecting local farming communities. Virus\u2010like particle (VLP) vaccines are highly regarded for their safety and efficacy. N\u2010glycosylation for stabilisation and recognition by antigen\u2010presenting cells has been a widely adopted strategy, particularly in enveloped viruses. Here, FMD virus (FMDV) VLPs were employed as a model for artificial glycosylation. N\u2010glycosylation was introduced by mutating the potential glycosylation site of VP1 and then N\u2010glycosylated FMDV VLPs were successfully produced in\n                    <jats:italic>Pichia pastoris<\/jats:italic>\n                    . Glycan profiling revealed that the majority of associated glycans (72.93%) were of the high\u2010mannose type, with additional hybrid type (4.16%) and complex type (22.92%) detected. Functional analyses demonstrated that glycosylation significantly enhanced the stability of VLPs and facilitated the uptake by antigen\u2010presenting cells. Animal experiments further revealed that glycosylation could induce a higher cellular immune response compared to WT VLPs, offering a reference for the glycosylation design of VLP vaccines.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70271","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T15:40:42Z","timestamp":1763998842000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glycosylated Foot\u2010And\u2010Mouth Disease Virus\u2010Like Particles Produced in\n                    <i>Pichia Pastoris<\/i>\n                    Enhance Stability and Immunogenicity"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7875-7304","authenticated-orcid":false,"given":"Zhiyao","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention College of Veterinary Medicine, Lanzhou University, 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