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This study reports the isolation and comprehensive characterisation of jumbo bacteriophage P19. It is a lytic phage with a long, contractile tail and is placed in the genus\n                    <jats:italic>Ceceduovirus<\/jats:italic>\n                    of the family\n                    <jats:italic>Straboviridae<\/jats:italic>\n                    . P19 possesses a large genome typical of jumbo phages, which likely contributes to its enhanced replication efficiency and adaptability. P19 exhibited strong lytic activity with a high efficiency of plating (EOP) against multiple\n                    <jats:italic>A.\u2009dhakensis<\/jats:italic>\n                    strains, a burst size of approximately 50 plaque\u2010forming units (PFU) per cell, and a latent period of 30\u2009min. It remained highly stable across pH\u20096\u201310 and temperatures up to 60\u00b0C. Genomic analysis revealed a 228\u2009kb genome with 414 open reading frames (ORFs) and absence of genes related to lysogeny, antibiotic resistance or virulence, indicating its potential for safe therapeutic and biocontrol applications. ORF_358 encodes a stable and soluble T4\u2010type lysozyme endolysin (molecular weight 18.8\u2009kDa) belonging to the glycoside hydrolase family 24. Molecular docking analysis demonstrated strong binding affinity (\u22126.4\u2009kcal\/mol) between P19_358 and the peptidoglycan NAG\u2010NAM dimer. Recombinant P19_358 endolysin exhibited 99.98% relative lytic activity against Gram\u2010positive bacterium\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacillus subtilis<\/jats:italic>\n                    <\/jats:styled-content>\n                    at a concentration of 75\u2009\u03bcg\/mL without EDTA pre\u2010treatment; whereas, it showed broad\u2010spectrum antibacterial activity against several Gram\u2010negative pathogens, including\n                    <jats:italic>A.\u2009dhakensis<\/jats:italic>\n                    ,\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Shigella flexneri<\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">Salmonella Typhimurium<\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">Pseudomonas aeruginosa<\/jats:styled-content>\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">Escherichia coli<\/jats:styled-content>\n                      ,\n                    <\/jats:italic>\n                    with relative activity ranging from 71% to 97% following pre\u2010treatment with 100\u2009mM EDTA. These findings collectively indicate that phage P19 and its endolysin P19_358 possess potent lytic activity and favourable stability profiles, supporting their development as promising antimicrobial agents against MDR Gram\u2010negative pathogens.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70289","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T05:29:35Z","timestamp":1767677375000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterisation of a Novel Jumbo Lytic\n                    <i>Aeromonas dhakensis<\/i>\n                    Bacteriophage\n                    <scp>P19<\/scp>\n                    and Its Endolysin"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0558-5402","authenticated-orcid":false,"given":"Farhat","family":"Ansari","sequence":"first","affiliation":[{"name":"Food Technology Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6556-8068","authenticated-orcid":false,"given":"Vandan","family":"Nagar","sequence":"additional","affiliation":[{"name":"Food Technology Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394621-8.00017-0"},{"key":"e_1_2_14_4_1","unstructured":"Andrews S.2010.\u201cFastQC: A Quality Control Tool for High Throughput Sequence Data.\u201dhttp:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/."},{"key":"e_1_2_14_5_1","volume-title":"Methods in Molecular Biology","author":"Baljinnyam B.","year":"2020"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13060465"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa977"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep08365"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01379-14"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14051016"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12090976"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01476"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12456"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00793"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2024.0212"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1454618"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020916"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00285-16"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv451"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)77022-8"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8010129"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10060310"},{"issue":"3","key":"e_1_2_14_25_1","article-title":"ResFinder \u2013 An Open Online Resource for Identification of Antimicrobial Resistance Genes in Next\u2010Generation Sequencing Data and Prediction of Phenotypes From Genotypes","volume":"8","author":"Florensa A. 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