{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:20:49Z","timestamp":1782742849015,"version":"3.54.5"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T00:00:00Z","timestamp":1766620800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82572635"],"award-info":[{"award-number":["82572635"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81925026"],"award-info":[{"award-number":["81925026"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82341218"],"award-info":[{"award-number":["82341218"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82130068"],"award-info":[{"award-number":["82130068"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82402710"],"award-info":[{"award-number":["82402710"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA0806400"],"award-info":[{"award-number":["2022YFA0806400"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024M751324"],"award-info":[{"award-number":["2024M751324"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Fusobacterium<\/jats:italic>\n , a gram\u2010negative anaerobic bacillus in mouth, gastrointestinal tract and elsewhere, has long been considered as opportunistic pathogen. Increasing evidence indicate the association of\n Fusobacterium<\/jats:italic>\n with human diseases, especially cancer. However, previous studies demonstrated contradictory prevalent features of\n Fusobacterium<\/jats:italic>\n species in normal and patient population. To address this dissonance, we developed a high\u2010precision multiplex PCR assay that allows concurrent identification of five species of\n Fusobacterium<\/jats:italic>\n (\n \n F.\u2009nucleatum<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n F.\u2009mortiferum<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n F.\u2009ulcerans<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n F.\u2009varium<\/jats:italic>\n <\/jats:styled-content>\n and\n \n F.\u2009necrophorum<\/jats:italic>\n <\/jats:styled-content>\n ) and four subspecies of\n \n F.\u2009nucleatum<\/jats:italic>\n <\/jats:styled-content>\n (\n nucleatum<\/jats:italic>\n ,\n animalis<\/jats:italic>\n ,\n vincentii<\/jats:italic>\n and\n polymorphum<\/jats:italic>\n ). By employing the PCR method, we investigated the prevalent features of\n Fusobacterium<\/jats:italic>\n communities in Southern Chinese population and cancer patients. Surprisingly, we found\n \n F.\u2009nucleatum<\/jats:italic>\n <\/jats:styled-content>\n was dominant in both Southern Chinese population and cancer patients, and discovered the correlations of\n Fusobacterium<\/jats:italic>\n species to host conditions. Moreover,\n \n F.\u2009mortiferum<\/jats:italic>\n <\/jats:styled-content>\n exhibited better diagnostic performance for cancers compared to other species, and the combination of\n \n F.\u2009mortiferum<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n F.\u2009nucleatum<\/jats:italic>\n <\/jats:styled-content>\n , body mass index and haemoglobin by a logistic regression model showed excellent diagnostic performances for cancers. Additionally, we determined the compositional features and loads of\n Fusobacterium<\/jats:italic>\n communities in paired tumour, adjacent tissues and normal tissues of colorectal cancer and lung cancer. Hence, we developed a high\u2010precision multiplex PCR assay to profile\n Fusobacterium<\/jats:italic>\n in human faeces and tumour, and demonstrate its prevalence in Southern Chinese population with correlations to host conditions and cancers.\n <\/jats:p>","DOI":"10.1111\/1751-7915.70292","type":"journal-article","created":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T02:56:46Z","timestamp":1766717806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Modified Method Incorporating Multiplex\n PCR<\/scp>\n Reveals\n Fusobacterium<\/i>\n Prevalence in Southern Chinese Population and Its Correlations in Cancers"],"prefix":"10.1111","volume":"18","author":[{"given":"Tingting","family":"Shen","sequence":"first","affiliation":[{"name":"Microbiome Medicine Center, Department of Laboratory Medicine Zhujiang Hospital, Southern Medical University Guangzhou People's Republic of China"},{"name":"Department of Laboratory Medicine Nanfang Hospital, Southern Medical University Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiarui","family":"Liang","sequence":"additional","affiliation":[{"name":"Microbiome Medicine Center, Department of Laboratory Medicine Zhujiang Hospital, Southern Medical University Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuyu","family":"Li","sequence":"additional","affiliation":[{"name":"Microbiome Medicine Center, Department of Laboratory Medicine Zhujiang Hospital, Southern Medical University Guangzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxie","family":"Xu","sequence":"additional","affiliation":[{"name":"Research Institute, DAAN Gene Co., Ltd. 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