{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:00:38Z","timestamp":1788300038104,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:00:00Z","timestamp":1773100800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82460297"],"award-info":[{"award-number":["82460297"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Antibiotic therapy is currently challenged by drug resistance and high recurrence in aerobic vaginitis (AV), making it urgent to seek novel strategies. In this study, we selected two potential probiotic strains\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lactobacillus crispatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    YBR\u201001 (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. crispatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    YBR\u201001) and\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    YBR\u201001 (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. plantarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    YBR\u201001) from our proprietary bacterial library, and comprehensively evaluated the protective potential against AV in mouse model induced by a pathogen cocktail. Supplementation with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. crispatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    YBR\u201001 and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. plantarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    YBR\u201001, either individually or in combination, attenuated vaginal edema, reduced proinflammatory mediators, and restored anti\u2010inflammatory cytokines, with the combination therapy yielding the best results. Mechanistically, the combination of strains significantly inhibited the TLR4\/MyD88\/NF\u2010\u03baB cascade (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), restored vaginal mucosal integrity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), and rebalanced the Th17\/Treg immunity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Moreover, given that postbiotics represent a safe and highly stable alternative strategy with emerging translational potential, the research investigated their impact derived from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. crispatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    YBR\u201001 and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. plantarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    YBR\u201001 on the phenotypic characteristics and molecular profile of the AV mouse model. While postbiotics could synergistically alleviate the AV\u2010like murine phenotype and improve inflammatory markers, the impacts were quantitatively similar as those observed with the live strain combination (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). More importantly, the postbiotics effectively mirrored the mechanisms of their live counterparts by downregulating the TLR4\/MyD88\/NF\u2010\u03baB pathway (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01), demonstrating the potential to enhance vaginal barrier (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01) and restoring Th17\/Treg balance (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), with the combination of postbiotics exhibiting superior advantages over monotherapy. Our study unveiled the therapeutic benefits of both potential probiotic\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    spp. and their postbiotics in AV, presenting an ideal prospect for clinical translation.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70327","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T04:24:59Z","timestamp":1773203099000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Vaginal\u2010Derived Potential Probiotics and Their Postbiotics Alleviate Aerobic Vaginitis via Suppressing\n                    <scp>TLR4<\/scp>\n                    \/\n                    <scp>MyD88<\/scp>\n                    \/\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    Signalling Pathway and Potentially Enhancing Vaginal Barrier"],"prefix":"10.1111","volume":"19","author":[{"given":"Meiyun","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University  Nanchang Jiangxi China"},{"name":"Department of Obstetrics and Gynecology The Sixth Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Queen Mary School, Jiangxi Medical College Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, Renji Hospital, School of Medicine Shanghai Jiao Tong University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"He","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Ninth Hospital in Nanchang  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wei","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buzhen","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0506-8536","authenticated-orcid":false,"given":"Tingtao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University  Nanchang Jiangxi China"},{"name":"National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Jiangxi Medical College Nanchang University  Nanchang Jiangxi China"},{"name":"Jiangxi Province Key Laboratory of Bioengineering Drugs, School of Pharmacy, Jiangxi Medical College Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,10]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2023.0490"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbl2805088"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112474"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602\u2010023\u201010050\u20100"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2022.11.004"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2021.106277"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21599"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1915254"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/1040841x.2022.2049696"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201055233\u20107"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010022\u201001337\u20105"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20100528.2002.00432.x"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2017.04.004"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010023\u201001594\u2010y"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564\u2010022\u201001083\u20102"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2024.09.014"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adq2732"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602\u2010024\u201010349\u20106"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanmic.2024.101037"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2019.1589281"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14798"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1226859"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.09.003"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2010.22"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1951155"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2023.2169858"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13010132"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12978\u2010021\u201001292\u20108"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12102444"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602\u2010017\u20109360\u20100"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2024.127787"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2024.100915"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.36740\/Merkur202305103"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575\u2010021\u201000440\u20106"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.20517\/mrr.2022.03"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12050843"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10091133"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.05.035"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2017.04.001"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2021.07.013"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.998813"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202202432"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010024\u201000521\u20109"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00460\u201013"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods12051118"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2025.116546"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201063110\u20100"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2022.115806"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.53105"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2012.12.037"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2024.127821"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2024.2448562"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010025\u201000813\u20108"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.07.029"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70327","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.70327","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T03:49:21Z","timestamp":1774669761000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":54,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/1751-7915.70327"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70327","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70327"}}