{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T06:20:18Z","timestamp":1774678818876,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:00:00Z","timestamp":1772928000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Candidal vaginitis is a prevalent inflammatory condition of the reproductive tract that has a substantial impact on women's health. Conventional antibiotic therapy frequently results in recurrence due to the non\u2010selective elimination of vaginal microbiota and subsequent dysbiosis. In order to overcome this limitation, the focus was directed towards traditional Chinese medicine, which has antimicrobial properties and it was here that pseudolaric acid B (PAB) was identified as a promising active monomer through the utilisation of virtual screening and bioinformatics approaches. In\u00a0vitro experiments confirmed that PAB inhibits biofilm formation, reduces ergosterol biosynthesis by downregulating the key gene\n                    <jats:italic>ERG11<\/jats:italic>\n                    , and enhances fungal cell membrane permeability, ultimately resulting in fungal cell death. In\u00a0vivo studies in a mouse model of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Candida albicans<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010induced vaginitis demonstrated that PAB exhibits superior therapeutic efficacy compared to conventional antibiotics. This is characterised by the restoration of normal tissue architecture, a reduction in inflammatory responses and a significant enrichment of beneficial vaginal microbiota as revealed by 16S rRNA sequencing. Collectively, these results highlight PAB as a promising dual\u2010mechanism antifungal agent which acts, at least in part, by disrupting fungal ergosterol biosynthesis and directly eradicating pathogens, while restoring vaginal microecological homeostasis.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70331","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:00:47Z","timestamp":1773032447000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pseudolaric Acid B Combats Drug\u2010Resistant\n                    <scp>\n                      <i>Candida albicans<\/i>\n                    <\/scp>\n                    Infection via Dual\u2010Action Mechanisms of Direct Antifungals and Vaginal Microbiota Restoration"],"prefix":"10.1111","volume":"19","author":[{"given":"Tianmeng","family":"Shao","sequence":"first","affiliation":[{"name":"Department of Microbiology and 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