{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:42:33Z","timestamp":1790653353353,"version":"4.1.0"},"reference-count":79,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002341","name":"Research Council of Finland","doi-asserted-by":"publisher","award":["355120"],"award-info":[{"award-number":["355120"]}],"id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hybrid vigour, or heterosis, is widely exploited in yeast strain improvement. Yet, how ploidy and genetic background jointly shape heterosis across industrially relevant stresses remains unclear. Here, we generated 1023\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    intraspecific hybrids derived from 18 genetically diverse parents, using two different approaches, yielding sets of hybrids with variable ploidy for the same parental combinations. High\u2010throughput growth assays in media with five stress conditions (14% ethanol, 1.5\u2009M NaCl, 0.15\u2009M lactic acid, 0.05\u2009M acetic acid, 0.05\u2009M HMF) revealed extensive heterosis across 6138 hybrid\u2010condition combinations. Most combinations displayed mid\u2010parent heterosis and over a third exceeded the best parent, with the strongest gains during growth in the presence of 14% ethanol and 1.5\u2009M NaCl. Increasing ploidy was generally associated with reduced growth and reduced best\u2010parent heterosis, whereas greater predicted hybrid heterozygosity or genetic distance between parents was positively associated with heterosis in the presence of 14% ethanol and 1.5\u2009M NaCl. Domestication status also affected these trends, as crosses between two domesticated strains tended to perform better in the presence of ethanol and NaCl, while crosses between two wild strains grew best in control conditions and in the presence of acetic acid. Together, these results demonstrate condition\u2010dependent contributions of ploidy and parentage to heterosis and provide targeted breeding strategies for the improvement of stress\u2010tolerance in industrial yeasts.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70337","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T05:17:13Z","timestamp":1774502233000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Influence of Ploidy and Genetic Background on Stress Tolerance of Intraspecific Yeast Hybrids"],"prefix":"10.1111","volume":"19","author":[{"given":"Kaisa","family":"Rinta\u2010Harri","sequence":"first","affiliation":[{"name":"VTT Technical Research Centre of Finland  Espoo Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tino","family":"Koponen","sequence":"additional","affiliation":[{"name":"VTT Technical Research Centre of Finland  Espoo Finland"},{"name":"Department of Bioproducts and Biosystems, School of Chemical Engineering Aalto University  Espoo Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Mojzita","sequence":"additional","affiliation":[{"name":"VTT Technical Research Centre of Finland  Espoo Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Jouhten","sequence":"additional","affiliation":[{"name":"Department of Bioproducts and Biosystems, School of Chemical Engineering Aalto University  Espoo Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianni","family":"Liti","sequence":"additional","affiliation":[{"name":"IRCAN CNRS, INSERM, C\u00f4te d'Azur University  Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7694-8277","authenticated-orcid":false,"given":"Kristoffer","family":"Krogerus","sequence":"additional","affiliation":[{"name":"VTT Technical Research Centre of Finland  Espoo Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/g3journal\/jkae286"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeb.13023"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859\u2010024\u201005817\u20103"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201300445"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201012041\u20108"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567\u20101364.2009.00583.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41559\u2010022\u201001671\u20109"},{"key":"e_1_2_11_10_1","volume-title":"Package \u2018agricolae\u2019 Title Statistical Procedures for Agricultural Research. 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