{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T05:09:42Z","timestamp":1778908182007,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Papaya leaf curl Guangdong virus<\/jats:italic>\n                    (PaLCuGDV), a member of the genus\n                    <jats:italic>Begomovirus<\/jats:italic>\n                    , poses a significant threat to passion fruit\n                    <jats:italic>\n                      (\n                      <jats:styled-content style=\"fixed-case\">Passiflora edulis<\/jats:styled-content>\n                    <\/jats:italic>\n                    ) cultivation. A recent field survey in the Rongjiang area of Guizhou Province revealed a high natural infection rate of PaLCuGDV (90.1%; 209\/232 plants). From a naturally infected passion fruit plant, the complete genome of a PaLCuGDV isolate was cloned and sequenced. The obtained genome, designated PaLCuGDV\u2010GZ (GenBank OP901623.1), comprises 2732 nucleotides and shows &gt;\u200990% similarity to other Chinese PaLCuGDV isolates based on BLAST analysis. To experimentally investigate its pathogenicity, an infectious clone containing a 1.4\u2010mer of the viral genome was constructed. Agroinoculation using this clone demonstrated that PaLCuGDV infection induced mild leaf wrinkling, curling, and chlorosis in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    , whereas it caused severe leaf curl and mosaic symptoms in passion fruit. The average experimental infection rate reached 46.7% in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. edulis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , while it was 96.7% in\n                    <jats:italic>N. benthamiana<\/jats:italic>\n                    at 4\u2009weeks post\u2010inoculation. Furthermore, transmission electron microscopy of\n                    <jats:italic>N. benthamiana<\/jats:italic>\n                    leaves infected with the infectious clone revealed two key findings: purified virions exhibited a unique \u201ctwin icosahedral\u201d structure, and infected cells showed a significant reduction in chloroplast number along with abnormal chloroplast morphology. In summary, this study reports the prevalence of PaLCuGDV in the field and successfully establishes an infectious clone, which provides an essential tool for future research into the gene function, pathogenicity, and virus\u2010host interactions of this economically important virus.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70339","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T04:21:28Z","timestamp":1777263688000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Construction and Biological Characterization of Papaya Leaf Curl Guangdong Virus Infectious cDNA Clone"],"prefix":"10.1111","volume":"19","author":[{"given":"Sheng","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural Microbiology, College of Agriculture Guizhou University  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaoli","family":"Wu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Green Pesticide Guizhou University  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoyao","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Microbiology, College of Agriculture Guizhou University  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengxi","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Microbiology, College of Agriculture Guizhou University  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9432-3983","authenticated-orcid":false,"given":"Xiangru","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agricultural Microbiology, College of Agriculture Guizhou University  Guiyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.22.7066"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.02.008"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2486.2008.01820.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2024.108812"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ery411"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2021.114888"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2025.113689"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83236-0"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3117"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1094\/PDIS-08-19-1619-PDN"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s42161-021-00932-z"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-053620160000100002"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2023.01.005"},{"issue":"9","key":"e_1_2_11_15_1","first-page":"1935","article-title":"Pathogenicity Analysis of Papaya Leaf Curl Guangdong Virus and Poinsettia Leaf Curl Virus","volume":"31","author":"Li J. 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