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In the present study, a chloroform respiring\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Dehalobacter restrictus<\/jats:italic>\n                    <\/jats:styled-content>\n                    strain was examined for its adaptability to a low affinity substrate (1,1\u2010dichloroethane) over five subcultures (~28 generations). We obtained an enhanced 11,1\u2010dichloroethane dechlorination rate by culturing with a 1:9 ratio of native (chloroform) to non\u2010native (1,1\u2010dichloroethane) substrates. We identified mutations corresponding to amino acids located in high\u2010entropy regions of the TmrA protein sequence, suggesting mutational plasticity at these sites. We revealed that the mutated TmrA structure showed increased binding affinity for 1,1\u2010dichloroethane by using molecular dynamics simulations and binding free energy computations. These findings provide insights into the adaptability of anaerobic organohalide\u2010respiring bacteria toward non\u2010native organohalogens and identify structural features of reductive dehalogenases that may be exploited for future enzyme engineering in bioremediation applications.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70353","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T12:09:22Z","timestamp":1777032562000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["From Energy Deprivation Stress to Catalytic Adaptation of Reductive Dehalogenase\n                    <scp>TmrA<\/scp>\n                    for 1,1\u2010\n                    <scp>DCA<\/scp>\n                    Dechlorination: An Experimental and in Silico Study"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0080-2500","authenticated-orcid":false,"given":"Yi","family":"Ren","sequence":"first","affiliation":[{"name":"Water Research Centre, School of Civil and Environmental Engineering University of New South Wales  Kensington Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan","family":"Landers","sequence":"additional","affiliation":[{"name":"Water Research Centre, School of Civil and Environmental Engineering University of New South Wales  Kensington Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Lee","sequence":"additional","affiliation":[{"name":"Water Research Centre, School of Civil and Environmental Engineering University of New South Wales  Kensington Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1880-0888","authenticated-orcid":false,"given":"Mike","family":"Manefield","sequence":"additional","affiliation":[{"name":"Water Research Centre, School of Civil and Environmental Engineering University of New South Wales  Kensington Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.softx.2015.06.001"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01512-18"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a026229"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.464913"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0026893308060150"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258118"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00803-24"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00732-24"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.2408420"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02312-13"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119306"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27072370"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.36.040306.132812"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2017.11.001"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2021.03.001"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00720-13"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12387"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1088"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.447079"},{"key":"e_1_2_12_21_1","volume-title":"Gaussian 09 Revision D.01","author":"Frisch M. 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