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Here, we demonstrate a nearly solvent\u2010free, mild aqueous strategy for the recovery of PHAs directly from wet bacterial biomass using crude enzyme systems derived from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Tenebrio molitor<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Under optimized conditions (0.6\u2009wt% crude protein, pH\u20097.6, 40\u00b0C), near\u2010quantitative (\u2265\u200995%) recovery of PHB and PHB\/HV and up to 60% recovery of mcl\u2010PHA were achieved without prior biomass drying. Proteomic analysis identified abundant digestive hydrolases, including \u03b1\u2010amylase and cathepsin and recombinant validation confirmed their contribution to polymer release, demonstrating that targeted enzyme combinations can substantially enhance mcl\u2010PHA recovery (up to 94%\u201398%). Gel permeation chromatography, NMR and thermal analysis demonstrated preservation of polymer molecular integrity and crystallinity comparable to chloroform extraction. Life cycle assessment revealed a three\u2010 to seven\u2010fold reduction in carbon footprint relative to conventional solvent and classical enzymatic methods, primarily due to elimination of drying and solvent use. These findings establish biologically driven biomass hydrolysis as a scalable downstream strategy and highlight insect\u2010derived enzyme systems as promising tools for integrated microbial biopolymer processing.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70360","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:10:36Z","timestamp":1777547436000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Tenebrio molitor<\/i>\n                    \u2010Derived Enzyme Systems Enable Solvent\u2010Reduced Recovery of Intracellular Polyhydroxyalkanoates"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5159-9461","authenticated-orcid":false,"given":"Sanja Skaro","family":"Bogojevic","sequence":"first","affiliation":[{"name":"Institute of Molecular Genetics and Genetic Engineering, University of Belgrade  Belgrade Republic of 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