{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T09:01:44Z","timestamp":1779958904896,"version":"3.53.1"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T00:00:00Z","timestamp":1779321600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100013288","name":"LIFE programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["860477"],"award-info":[{"award-number":["860477"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Microbial Biotechnology"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Grass silage fed green biorefineries require high quality grass silage leachates to produce high quality products. Real\u2010time, cost\u2010effective methods to monitor key leachate analytes are needed for small\u2010scale decentralised systems. In this study,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    mutants, designated JSP0090 and JSP0094, were created to quantify\n                    <jats:sc>d<\/jats:sc>\n                    \u2010 and\n                    <jats:sc>l<\/jats:sc>\n                    \u2010lactic acid concentrations in grass leachate samples using an oxygen probe. The genes encoding either\n                    <jats:sc>d<\/jats:sc>\n                    \u2010 or\n                    <jats:sc>l<\/jats:sc>\n                    \u2010lactate dehydrogenase were expressed in an exclusion biosensor strain (JSK0115). This strain required successive gene deletions to prevent interference from sugars, amino acids and organic acids present in grass silage leachates. This strain was incapable of catabolising\n                    <jats:sc>d<\/jats:sc>\n                    \u2010lactic acid,\n                    <jats:sc>l<\/jats:sc>\n                    \u2010lactic acid, acetic acid, propionic acid, formic acid, ethanol, glucose, fructose,\n                    <jats:sc>l<\/jats:sc>\n                    \u2010alanine, VFAs, glycerol, mannitol and succinate. Gene knockouts were achieved using P1 phage lysates and CRISPR Cas 9 methods to target key steps in the catabolism of these compounds, with the exception of succinate. For this metabolite, the di\u2010carboxylic acid transporters YaaH, DctA, YchM, DcuA and DcuB had to be deleted. The effectiveness of the biosensors for selectively measuring\n                    <jats:sc>d\u2010<\/jats:sc>\n                    and\n                    <jats:sc>l<\/jats:sc>\n                    \u2010lactic acid was assessed in Austrian and Irish grass silage leachate. The concentrations measured were comparable to those obtained using a commercial enzyme kit.\n                  <\/jats:p>","DOI":"10.1111\/1751-7915.70374","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T05:19:38Z","timestamp":1779427178000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Stereospecific Lactic Acid Exclusion Biosensor for Grass Silage Fed Green Biorefinery"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5032-9961","authenticated-orcid":false,"given":"Roderick N.","family":"van Roosmalen","sequence":"first","affiliation":[{"name":"School of Biosystems and Food Engineering University College Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2137-3338","authenticated-orcid":false,"given":"Cormac D.","family":"Murphy","sequence":"additional","affiliation":[{"name":"School of Biomolecular and Biomedical Science University College Dublin  Dublin 4 Ireland"},{"name":"Conway Institute University College Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8137-8336","authenticated-orcid":false,"given":"Joseph B.","family":"Sweeney","sequence":"additional","affiliation":[{"name":"School of Biosystems and Food Engineering University College Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-018-00222-4"},{"key":"e_1_2_10_3_1","unstructured":"BioRefine.2024.\u201cBioRefine\u2014Den gr\u00f8nne l\u00f8sning.\u201dhttps:\/\/biorefine.dk\/."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2015.04.128"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky354"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2020.114401"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.18.5624-5635.1999"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2012.05.027"},{"key":"e_1_2_10_10_1","unstructured":"Farm4More.2019.\u201cLIFE Farm4More \u2010 Future Agricultural Management for multiple outputs on climate and rural development.\u201dhttps:\/\/farm4more.eu\/. Accessed on 17 May 2026."},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80284-8"},{"issue":"1","key":"e_1_2_10_12_1","first-page":"147","article-title":"The Green Biorefinery Concept: Optimal Plant Locations and Sizes for Austria","volume":"21","author":"H\u00f6ltinger S.","year":"2012","journal-title":"Jahrbuch der \u00d6GA (\u00d6sterreichische Gesellschaft f\u00fcr Agrar\u00f6konomie)"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/gfs.12594"},{"issue":"1","key":"e_1_2_10_14_1","first-page":"8","article-title":"The Green Biorefinery Austria\u2010Development of an Integrated System for Green Biomass Utilization","volume":"18","author":"Kromus S.","year":"2004","journal-title":"Chemical and Biochemical Engineering Quarterly"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bbb.219"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1533\/9780857097385.1.335"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c04289"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2015.06.011"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.24.7679-7686.1997"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep15096"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bbb.1957"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731119002970"},{"key":"e_1_2_10_23_1","unstructured":"Schaffenberger M.2013.\u201cGreen Biorefinery\u2014The Production Isolation and Polishing of Amino Acids From Grass Silage Juice at Pilot and Laboratory Scale.\u201dUnpublished thesis (PhD) TU Graz."},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2018.08.064"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2022.105128"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6887-4"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2022.110091"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.6.1796-1800.1994"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107022200"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00425614"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.17737\/tre.2015.1.1.008"}],"container-title":["Microbial Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70374","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/1751-7915.70374","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/1751-7915.70374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T08:03:05Z","timestamp":1779955385000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/1751-7915.70374"],"URL":"https:\/\/doi.org\/10.1111\/1751-7915.70374","archive":["Portico"],"relation":{},"ISSN":["1751-7915","1751-7915"],"issn-type":[{"value":"1751-7915","type":"print"},{"value":"1751-7915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2026-02-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70374"}}